Study on the effect of perfusion pressure on decellularisation of porcine oesophagi

• Main Author: Tan, Jermaine
• Other Authors: Chian Kerm Sin
• Format: Final Year Project
• Language: English
• Published: Nanyang Technological University 2021
• Subjects: Engineering::Bioengineering; Engineering::Mechanical engineering; Engineering::Materials
• Online Access: https://hdl.handle.net/10356/149538
• Institution: Nanyang Technological University

With huge advancements in recent years, tissue engineering has 3 main components, namely: 1. Reparative cells that can form an extracellular matrix (ECM) 2. A suitable scaffold for cell transplantation 3. Bio-reactive molecules to promote growth and cell regeneration This study focused on attaining the best possible ECM for tissue regeneration of the oesophagus. The method investigated to achieve successful decellularisation is via Sodium Dodecyl Sulfate (SDS) perfusion pressure decellularisation of the porcine oesophagus. This research paper addresses certain problems faced during the decellularisation process, such as preserving the native folded structures within the oesophagus throughout decellularisation and selecting an alternate storage solution to protect the fresh oesophagi from contaminants before decellularisation. Results from this study showed the need for a low flowrate difference within the inlet and outlet tubing to maintain a closed lumen structure. It also revealed a possible relationship between the reduction of the decellularisation duration and an increased inflow rate.