The anammox process at typical feast-famine states : reactor performance, sludge activity and microbial community

Anaerobic ammonium oxidation (Anammox) is a chemolithotrophic bioprocess which has been widely applied in the treatment of different concentrations of ammonium-containing wastewaters. However, there is less attention on the problem that the instantaneous growth rate (or metabolic rate) and equilibri...

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Main Authors: Kang, Da, Yu, Tao, Xu, Dongdong, Zeng, Zhuo, Ding, Aqiang, Zhang, Meng, Shan, Shengdao, Zhang, Wudi, Zheng, Ping
Other Authors: Nanyang Environment and Water Research Institute
Format: Article
Language:English
Published: 2021
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Online Access:https://hdl.handle.net/10356/150424
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-1504242021-08-04T08:19:24Z The anammox process at typical feast-famine states : reactor performance, sludge activity and microbial community Kang, Da Yu, Tao Xu, Dongdong Zeng, Zhuo Ding, Aqiang Zhang, Meng Shan, Shengdao Zhang, Wudi Zheng, Ping Nanyang Environment and Water Research Institute Advanced Environmental Biotechnology Centre (AEBC) Engineering::Environmental engineering Anammox Process Feast-famine State Anaerobic ammonium oxidation (Anammox) is a chemolithotrophic bioprocess which has been widely applied in the treatment of different concentrations of ammonium-containing wastewaters. However, there is less attention on the problem that the instantaneous growth rate (or metabolic rate) and equilibrium growth rate were un-synchronous for anammox bacteria due to their long generation time and self-immobilization of the granular sludge which could lead to the inaccurate estimation. In this study, the anammox process was firstly divided to four typical feast-famine (starvation, satiation, tolerance and poison) states based on the combination of both off-site and in-situ anammox reaction kinetics. Then, four respective lab-scale bioreactors were operated at each state for over a year to achieve stable anammox performance. The results showed that the nitrogen removal rates of bioreactors were 0.53, 2.24, 9.30 and 12.96 kg N/(m³·d); and the specific anammox activities of granular sludge were 188.94 (48%), 313.29 (79%), 397.50 (100%) and 198.60 (50%) mg N/(g VSS·d) which could reflect the reactivity of each feast-famine state. The stable microbial communities of bioreactors were cultured and analyzed, whose species diversity went down with the decrease of Shannon and ACE index. The relative abundance of anammox bacteria increased from 11% to 57% from starvation to poison state. Candidatus Brocadia/Nitrospira, Candidatus Kuenenia and Brocadiaceae unclassified were revealed to be the distinctive functional bacteria, which could serve as the indicator of each state. The setting up of the typical feast-famine states could be regarded as the landmark to help the design, control and optimization of anammox process. This research was financially supported by the National Natural Science Foundation of China (51578484 and 51778563) and Research Funds for Central Universities (2017xzzx010-03). Major Scientific and Technological Specialized Project of Zhejiang Province (2015C03013) and Key Research and Development program of Zhejiang Province (2018C03031) were also gratefully thanked. 2021-08-04T08:19:24Z 2021-08-04T08:19:24Z 2019 Journal Article Kang, D., Yu, T., Xu, D., Zeng, Z., Ding, A., Zhang, M., Shan, S., Zhang, W. & Zheng, P. (2019). The anammox process at typical feast-famine states : reactor performance, sludge activity and microbial community. Chemical Engineering Journal, 370, 110-119. https://dx.doi.org/10.1016/j.cej.2019.03.111 1385-8947 https://hdl.handle.net/10356/150424 10.1016/j.cej.2019.03.111 2-s2.0-85063263254 370 110 119 en Chemical Engineering Journal © 2019 Elsevier B.V. All rights reserved.
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Environmental engineering
Anammox Process
Feast-famine State
spellingShingle Engineering::Environmental engineering
Anammox Process
Feast-famine State
Kang, Da
Yu, Tao
Xu, Dongdong
Zeng, Zhuo
Ding, Aqiang
Zhang, Meng
Shan, Shengdao
Zhang, Wudi
Zheng, Ping
The anammox process at typical feast-famine states : reactor performance, sludge activity and microbial community
description Anaerobic ammonium oxidation (Anammox) is a chemolithotrophic bioprocess which has been widely applied in the treatment of different concentrations of ammonium-containing wastewaters. However, there is less attention on the problem that the instantaneous growth rate (or metabolic rate) and equilibrium growth rate were un-synchronous for anammox bacteria due to their long generation time and self-immobilization of the granular sludge which could lead to the inaccurate estimation. In this study, the anammox process was firstly divided to four typical feast-famine (starvation, satiation, tolerance and poison) states based on the combination of both off-site and in-situ anammox reaction kinetics. Then, four respective lab-scale bioreactors were operated at each state for over a year to achieve stable anammox performance. The results showed that the nitrogen removal rates of bioreactors were 0.53, 2.24, 9.30 and 12.96 kg N/(m³·d); and the specific anammox activities of granular sludge were 188.94 (48%), 313.29 (79%), 397.50 (100%) and 198.60 (50%) mg N/(g VSS·d) which could reflect the reactivity of each feast-famine state. The stable microbial communities of bioreactors were cultured and analyzed, whose species diversity went down with the decrease of Shannon and ACE index. The relative abundance of anammox bacteria increased from 11% to 57% from starvation to poison state. Candidatus Brocadia/Nitrospira, Candidatus Kuenenia and Brocadiaceae unclassified were revealed to be the distinctive functional bacteria, which could serve as the indicator of each state. The setting up of the typical feast-famine states could be regarded as the landmark to help the design, control and optimization of anammox process.
author2 Nanyang Environment and Water Research Institute
author_facet Nanyang Environment and Water Research Institute
Kang, Da
Yu, Tao
Xu, Dongdong
Zeng, Zhuo
Ding, Aqiang
Zhang, Meng
Shan, Shengdao
Zhang, Wudi
Zheng, Ping
format Article
author Kang, Da
Yu, Tao
Xu, Dongdong
Zeng, Zhuo
Ding, Aqiang
Zhang, Meng
Shan, Shengdao
Zhang, Wudi
Zheng, Ping
author_sort Kang, Da
title The anammox process at typical feast-famine states : reactor performance, sludge activity and microbial community
title_short The anammox process at typical feast-famine states : reactor performance, sludge activity and microbial community
title_full The anammox process at typical feast-famine states : reactor performance, sludge activity and microbial community
title_fullStr The anammox process at typical feast-famine states : reactor performance, sludge activity and microbial community
title_full_unstemmed The anammox process at typical feast-famine states : reactor performance, sludge activity and microbial community
title_sort anammox process at typical feast-famine states : reactor performance, sludge activity and microbial community
publishDate 2021
url https://hdl.handle.net/10356/150424
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