Human Wharton's jelly mesenchymal stem cells show unique gene expression compared with bone marrow mesenchymal stem cells using single-cell RNA-sequencing

Human Wharton's jelly stem cells (hWJSCs) isolated from the human umbilical cord are a unique population of mesenchymal stem cells (MSCs) with significant clinical utility. Their broad differentiation potential, high rate of proliferation, ready availability from discarded cords, and prolonged...

Full description

Saved in:
Bibliographic Details
Main Authors: Barrett, Angela N., Fong, Chui-Yee, Subramanian, Arjunan, Liu, Wenting, Feng, Yirui, Choolani, Mahesh, Biswas, Arijit, Rajapakse, Jagath Chandana, Bongso, Ariff
Other Authors: School of Computer Science and Engineering
Format: Article
Language:English
Published: 2021
Subjects:
Online Access:https://hdl.handle.net/10356/150799
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Nanyang Technological University
Language: English
id sg-ntu-dr.10356-150799
record_format dspace
spelling sg-ntu-dr.10356-1507992021-06-14T02:44:43Z Human Wharton's jelly mesenchymal stem cells show unique gene expression compared with bone marrow mesenchymal stem cells using single-cell RNA-sequencing Barrett, Angela N. Fong, Chui-Yee Subramanian, Arjunan Liu, Wenting Feng, Yirui Choolani, Mahesh Biswas, Arijit Rajapakse, Jagath Chandana Bongso, Ariff School of Computer Science and Engineering Engineering::Computer science and engineering Wharton's Jelly Stem Cells Single Cell Human Wharton's jelly stem cells (hWJSCs) isolated from the human umbilical cord are a unique population of mesenchymal stem cells (MSCs) with significant clinical utility. Their broad differentiation potential, high rate of proliferation, ready availability from discarded cords, and prolonged maintenance of stemness properties in culture make them an attractive alternative source of MSCs with therapeutic value compared with human bone marrow MSCs (hBMMSCs). We aimed to characterize the differences in gene expression profiles between these two stem cell types using single-cell RNA sequencing (scRNA-Seq) to determine which pathways are involved in conferring hWJSCs with their unique properties. We identified 436 significantly differentially expressed genes between the two cell types, playing roles in processes, including immunomodulation, angiogenesis, wound healing, apoptosis, antitumor activity, and chemotaxis. Expression of immune molecules is particularly high in hWJSCs compared with hBMMSCs. These differences in gene expression may help to explain many of the advantages that hWJSCs have over hBMMSCs for clinical application. Although cell surface protein marker expression indicates that isolated hWJSCs and hBMMSCs are both homogenous populations, using scRNA-Seq we can clearly identify extreme variability in expression levels between individual cells within a certain cell type. If the cells are examined as bulk populations, it is not possible to appreciate that a single cell may be making a major unique contribution to the apparent overall expression level. We demonstrated how the fine tuning of expression within hWJSCs and hBMMSCs may be achieved by expression of molecules with opposing function between two cells. We hypothesize that a greater understanding of these differences in gene expression between the two cell types may aid in the development of new therapies using hWJSCs. This work was supported by the Singapore National University Health System (NUHS) Aspiration Fund (New Idea; R-174-000-155-720) research grant. 2021-06-14T02:44:43Z 2021-06-14T02:44:43Z 2019 Journal Article Barrett, A. N., Fong, C., Subramanian, A., Liu, W., Feng, Y., Choolani, M., Biswas, A., Rajapakse, J. C. & Bongso, A. (2019). Human Wharton's jelly mesenchymal stem cells show unique gene expression compared with bone marrow mesenchymal stem cells using single-cell RNA-sequencing. Stem Cells and Development, 28(3), 196-211. https://dx.doi.org/10.1089/scd.2018.0132 1547-3287 https://hdl.handle.net/10356/150799 10.1089/scd.2018.0132 30484393 2-s2.0-85061054613 3 28 196 211 en Stem Cells and Development © 2019 Mary Ann Liebert, Inc., publishers. All rights reserved.
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Computer science and engineering
Wharton's Jelly Stem Cells
Single Cell
spellingShingle Engineering::Computer science and engineering
Wharton's Jelly Stem Cells
Single Cell
Barrett, Angela N.
Fong, Chui-Yee
Subramanian, Arjunan
Liu, Wenting
Feng, Yirui
Choolani, Mahesh
Biswas, Arijit
Rajapakse, Jagath Chandana
Bongso, Ariff
Human Wharton's jelly mesenchymal stem cells show unique gene expression compared with bone marrow mesenchymal stem cells using single-cell RNA-sequencing
description Human Wharton's jelly stem cells (hWJSCs) isolated from the human umbilical cord are a unique population of mesenchymal stem cells (MSCs) with significant clinical utility. Their broad differentiation potential, high rate of proliferation, ready availability from discarded cords, and prolonged maintenance of stemness properties in culture make them an attractive alternative source of MSCs with therapeutic value compared with human bone marrow MSCs (hBMMSCs). We aimed to characterize the differences in gene expression profiles between these two stem cell types using single-cell RNA sequencing (scRNA-Seq) to determine which pathways are involved in conferring hWJSCs with their unique properties. We identified 436 significantly differentially expressed genes between the two cell types, playing roles in processes, including immunomodulation, angiogenesis, wound healing, apoptosis, antitumor activity, and chemotaxis. Expression of immune molecules is particularly high in hWJSCs compared with hBMMSCs. These differences in gene expression may help to explain many of the advantages that hWJSCs have over hBMMSCs for clinical application. Although cell surface protein marker expression indicates that isolated hWJSCs and hBMMSCs are both homogenous populations, using scRNA-Seq we can clearly identify extreme variability in expression levels between individual cells within a certain cell type. If the cells are examined as bulk populations, it is not possible to appreciate that a single cell may be making a major unique contribution to the apparent overall expression level. We demonstrated how the fine tuning of expression within hWJSCs and hBMMSCs may be achieved by expression of molecules with opposing function between two cells. We hypothesize that a greater understanding of these differences in gene expression between the two cell types may aid in the development of new therapies using hWJSCs.
author2 School of Computer Science and Engineering
author_facet School of Computer Science and Engineering
Barrett, Angela N.
Fong, Chui-Yee
Subramanian, Arjunan
Liu, Wenting
Feng, Yirui
Choolani, Mahesh
Biswas, Arijit
Rajapakse, Jagath Chandana
Bongso, Ariff
format Article
author Barrett, Angela N.
Fong, Chui-Yee
Subramanian, Arjunan
Liu, Wenting
Feng, Yirui
Choolani, Mahesh
Biswas, Arijit
Rajapakse, Jagath Chandana
Bongso, Ariff
author_sort Barrett, Angela N.
title Human Wharton's jelly mesenchymal stem cells show unique gene expression compared with bone marrow mesenchymal stem cells using single-cell RNA-sequencing
title_short Human Wharton's jelly mesenchymal stem cells show unique gene expression compared with bone marrow mesenchymal stem cells using single-cell RNA-sequencing
title_full Human Wharton's jelly mesenchymal stem cells show unique gene expression compared with bone marrow mesenchymal stem cells using single-cell RNA-sequencing
title_fullStr Human Wharton's jelly mesenchymal stem cells show unique gene expression compared with bone marrow mesenchymal stem cells using single-cell RNA-sequencing
title_full_unstemmed Human Wharton's jelly mesenchymal stem cells show unique gene expression compared with bone marrow mesenchymal stem cells using single-cell RNA-sequencing
title_sort human wharton's jelly mesenchymal stem cells show unique gene expression compared with bone marrow mesenchymal stem cells using single-cell rna-sequencing
publishDate 2021
url https://hdl.handle.net/10356/150799
_version_ 1703971239854342144