Enhanced performance of chitosan/keratin membranes with potential application in peripheral nerve repair

Although surgical management of peripheral nerve injuries (PNIs) has improved over time, autografts are still the current "gold standard" treatment for PNIs, which presents numerous limitations. In an attempt to improve natural biomaterial-based nerve guidance conduits (NGCs), chitosan (CH...

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Main Authors: Carvalho, Cristiana R., Costa, João B., Costa, Lígia, Silva-Correia, Joana, Moay, Zi Kuang, Ng, Kee Woei, Reis, Rui L., Oliveira, Joaquim M.
Other Authors: School of Materials Science and Engineering
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Language:English
Published: 2021
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Online Access:https://hdl.handle.net/10356/151531
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spelling sg-ntu-dr.10356-1515312022-07-21T08:05:31Z Enhanced performance of chitosan/keratin membranes with potential application in peripheral nerve repair Carvalho, Cristiana R. Costa, João B. Costa, Lígia Silva-Correia, Joana Moay, Zi Kuang Ng, Kee Woei Reis, Rui L. Oliveira, Joaquim M. School of Materials Science and Engineering Skin Research Institute of Singapore Nanyang Environment and Water Research Institute Environmental Chemistry and Materials Centre Engineering::Materials Hair Keratin Hydrogel Cellular-response Although surgical management of peripheral nerve injuries (PNIs) has improved over time, autografts are still the current "gold standard" treatment for PNIs, which presents numerous limitations. In an attempt to improve natural biomaterial-based nerve guidance conduits (NGCs), chitosan (CHT), a derivative of the naturally occurring biopolymer chitin, has been explored for peripheral nerve regeneration (PNR). In addition to CHT, keratin has gained enormous attention as a biomaterial and tissue engineering scaffolding. In this study, biomimetic CHT/keratin membranes were produced using a solvent casting technique. These membranes were broadly characterized in terms of their surface topography and physicochemical properties, with techniques such as Fourier Transform Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC), contact angle, weight loss and water uptake measurements, Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM). Biological in vitro assays were also performed, where a preliminary cytotoxicity screening with the L929 fibroblast cell line revealed that the membranes and respective materials are suitable for cell culture. In addition, Schwann cells, fibroblasts and endothelial cells were directly seeded in the membranes. Quantitative and qualitative assays revealed that the addition of keratin enhanced cell viablity and adhesion. Based on the encouraging in vitro results, the in vivo angiogenic/antiangiogenic potential of CHT and CHT/keratin membranes was assessed, using an optimized chick embryo chorioallantoic membrane assay, where higher angiogenic responses were seen in keratin-enriched materials. Overall, the obtained results indicate the higher potential of CHT/keratin membranes for guided tissue regeneration applications in the field of PNR. The authors are thankful for the chitosan raw material provided by Altakitin S.A. (Lisboa, Portugal). This study was also supported by the European Community’s Seventh Framework Programme (FP7-HEALTH-2011) under grant agreement no. 278612 (BIOHYBRID). The authors acknowledge the Portuguese Foundation for Science and Technology (FCT) for the financial support provided to Joaquim M. Oliveira (IF/ 00423/2012 and IF/01285/2015) and Joana Silva-Correia (IF/ 00115/2015) under the program “Investigador FCT”. The authors would also like to acknowledge the contribution of Elsa Ribeiro for the SEM image acquisition; Sandra Pina for the acquisition of XRD diffractograms; and Teresa Oliveira for histology sample processing. 2021-06-18T05:46:35Z 2021-06-18T05:46:35Z 2019 Journal Article Carvalho, C. R., Costa, J. B., Costa, L., Silva-Correia, J., Moay, Z. K., Ng, K. W., Reis, R. L. & Oliveira, J. M. (2019). Enhanced performance of chitosan/keratin membranes with potential application in peripheral nerve repair. Biomaterials Science, 7(12), 5451-5466. https://dx.doi.org/10.1039/c9bm01098j 2047-4849 https://hdl.handle.net/10356/151531 10.1039/c9bm01098j 31642822 2-s2.0-85075303143 12 7 5451 5466 en Biomaterials Science © 2019 The Royal Society of Chemistry. All rights reserved.
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Materials
Hair Keratin Hydrogel
Cellular-response
spellingShingle Engineering::Materials
Hair Keratin Hydrogel
Cellular-response
Carvalho, Cristiana R.
Costa, João B.
Costa, Lígia
Silva-Correia, Joana
Moay, Zi Kuang
Ng, Kee Woei
Reis, Rui L.
Oliveira, Joaquim M.
Enhanced performance of chitosan/keratin membranes with potential application in peripheral nerve repair
description Although surgical management of peripheral nerve injuries (PNIs) has improved over time, autografts are still the current "gold standard" treatment for PNIs, which presents numerous limitations. In an attempt to improve natural biomaterial-based nerve guidance conduits (NGCs), chitosan (CHT), a derivative of the naturally occurring biopolymer chitin, has been explored for peripheral nerve regeneration (PNR). In addition to CHT, keratin has gained enormous attention as a biomaterial and tissue engineering scaffolding. In this study, biomimetic CHT/keratin membranes were produced using a solvent casting technique. These membranes were broadly characterized in terms of their surface topography and physicochemical properties, with techniques such as Fourier Transform Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC), contact angle, weight loss and water uptake measurements, Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM). Biological in vitro assays were also performed, where a preliminary cytotoxicity screening with the L929 fibroblast cell line revealed that the membranes and respective materials are suitable for cell culture. In addition, Schwann cells, fibroblasts and endothelial cells were directly seeded in the membranes. Quantitative and qualitative assays revealed that the addition of keratin enhanced cell viablity and adhesion. Based on the encouraging in vitro results, the in vivo angiogenic/antiangiogenic potential of CHT and CHT/keratin membranes was assessed, using an optimized chick embryo chorioallantoic membrane assay, where higher angiogenic responses were seen in keratin-enriched materials. Overall, the obtained results indicate the higher potential of CHT/keratin membranes for guided tissue regeneration applications in the field of PNR.
author2 School of Materials Science and Engineering
author_facet School of Materials Science and Engineering
Carvalho, Cristiana R.
Costa, João B.
Costa, Lígia
Silva-Correia, Joana
Moay, Zi Kuang
Ng, Kee Woei
Reis, Rui L.
Oliveira, Joaquim M.
format Article
author Carvalho, Cristiana R.
Costa, João B.
Costa, Lígia
Silva-Correia, Joana
Moay, Zi Kuang
Ng, Kee Woei
Reis, Rui L.
Oliveira, Joaquim M.
author_sort Carvalho, Cristiana R.
title Enhanced performance of chitosan/keratin membranes with potential application in peripheral nerve repair
title_short Enhanced performance of chitosan/keratin membranes with potential application in peripheral nerve repair
title_full Enhanced performance of chitosan/keratin membranes with potential application in peripheral nerve repair
title_fullStr Enhanced performance of chitosan/keratin membranes with potential application in peripheral nerve repair
title_full_unstemmed Enhanced performance of chitosan/keratin membranes with potential application in peripheral nerve repair
title_sort enhanced performance of chitosan/keratin membranes with potential application in peripheral nerve repair
publishDate 2021
url https://hdl.handle.net/10356/151531
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