Autoinducer-2-mediated quorum sensing partially regulates the toxic shock response of anaerobic digestion

This study discovered a strong correlation between the autoinducer-2 (AI-2)-mediated quorum sensing (QS) with the performance of a submerged anaerobic membrane bioreactor during its recovery from a pentachlorophenol (PCP) shock: a decrease in AI-2 levels coincided with a reduction in volatile fatty...

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Main Authors: Xiao, Yeyuan, Yaohari, Hazarki, Zhou, Zhongbo, Sze, Chun Chau, Stuckey, David C.
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2021
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Online Access:https://hdl.handle.net/10356/151702
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-1517022021-06-24T06:18:00Z Autoinducer-2-mediated quorum sensing partially regulates the toxic shock response of anaerobic digestion Xiao, Yeyuan Yaohari, Hazarki Zhou, Zhongbo Sze, Chun Chau Stuckey, David C. School of Biological Sciences Nanyang Environment and Water Research Institute Advanced Environmental Biotechnology Centre (AEBC) Science::Biological sciences Quorum Sensing Anaerobic Digestion This study discovered a strong correlation between the autoinducer-2 (AI-2)-mediated quorum sensing (QS) with the performance of a submerged anaerobic membrane bioreactor during its recovery from a pentachlorophenol (PCP) shock: a decrease in AI-2 levels coincided with a reduction in volatile fatty acid concentrations, and corresponded significantly to a decrease in the relative abundance of Firmicutes, and to an increase in the relative abundance of Bacteroidetes and Synergistetes. Further batch experiments with the addition of an AI-2-regulating Escherichia coli mutant culture showed that a reduction in AI-2 levels resulted in the highest biogas production rate during a PCP shock. In contrast, an increase in AI-2 levels via addition of the E. coli wild type strain or an AI-2 precursor showed no obvious effects on biogas production. These results suggest that the AI-2 level in anaerobic sludge was governed primarily by Firmicutes, and the AI-2-mediated QS partially regulates the toxic shock response of anaerobic sludge via tuning the activities of Firmicutes and Synergistetes. A decrease in the AI-2 level might reduce acetogenesis and favor hydrogenotrophic methanogenesis, thus resulting in less VFA accumulation and higher methane production during the PCP shock. This study is the first of this type that exploits the role of quorum sensing in the toxic shock response of anaerobic sludge; it demonstrates a novel approach to shortening the recovery period of anaerobic processes via manipulating the AI-2-mediated QS. National Research Foundation (NRF) The project was supported and administrated by the Environment & Water Industry Programme Office (EWI) for and on behalf of the National Research Foundation Board (NRF), Singapore (EWI-IRIS-0807). The project was also supported by the Shantou University Scientific Research Foundation for Talents (No.: NTF16015) and the National Natural Science Foundation of China (No.: 51750110514). 2021-06-24T06:18:00Z 2021-06-24T06:18:00Z 2019 Journal Article Xiao, Y., Yaohari, H., Zhou, Z., Sze, C. C. & Stuckey, D. C. (2019). Autoinducer-2-mediated quorum sensing partially regulates the toxic shock response of anaerobic digestion. Water Research, 158, 94-105. https://dx.doi.org/10.1016/j.watres.2019.04.024 0043-1354 0000-0001-5694-5319 https://hdl.handle.net/10356/151702 10.1016/j.watres.2019.04.024 31022531 2-s2.0-85064818872 158 94 105 en EWI-IRIS-0807 Water Research © 2019 Elsevier Ltd. All rights reserved.
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Biological sciences
Quorum Sensing
Anaerobic Digestion
spellingShingle Science::Biological sciences
Quorum Sensing
Anaerobic Digestion
Xiao, Yeyuan
Yaohari, Hazarki
Zhou, Zhongbo
Sze, Chun Chau
Stuckey, David C.
Autoinducer-2-mediated quorum sensing partially regulates the toxic shock response of anaerobic digestion
description This study discovered a strong correlation between the autoinducer-2 (AI-2)-mediated quorum sensing (QS) with the performance of a submerged anaerobic membrane bioreactor during its recovery from a pentachlorophenol (PCP) shock: a decrease in AI-2 levels coincided with a reduction in volatile fatty acid concentrations, and corresponded significantly to a decrease in the relative abundance of Firmicutes, and to an increase in the relative abundance of Bacteroidetes and Synergistetes. Further batch experiments with the addition of an AI-2-regulating Escherichia coli mutant culture showed that a reduction in AI-2 levels resulted in the highest biogas production rate during a PCP shock. In contrast, an increase in AI-2 levels via addition of the E. coli wild type strain or an AI-2 precursor showed no obvious effects on biogas production. These results suggest that the AI-2 level in anaerobic sludge was governed primarily by Firmicutes, and the AI-2-mediated QS partially regulates the toxic shock response of anaerobic sludge via tuning the activities of Firmicutes and Synergistetes. A decrease in the AI-2 level might reduce acetogenesis and favor hydrogenotrophic methanogenesis, thus resulting in less VFA accumulation and higher methane production during the PCP shock. This study is the first of this type that exploits the role of quorum sensing in the toxic shock response of anaerobic sludge; it demonstrates a novel approach to shortening the recovery period of anaerobic processes via manipulating the AI-2-mediated QS.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Xiao, Yeyuan
Yaohari, Hazarki
Zhou, Zhongbo
Sze, Chun Chau
Stuckey, David C.
format Article
author Xiao, Yeyuan
Yaohari, Hazarki
Zhou, Zhongbo
Sze, Chun Chau
Stuckey, David C.
author_sort Xiao, Yeyuan
title Autoinducer-2-mediated quorum sensing partially regulates the toxic shock response of anaerobic digestion
title_short Autoinducer-2-mediated quorum sensing partially regulates the toxic shock response of anaerobic digestion
title_full Autoinducer-2-mediated quorum sensing partially regulates the toxic shock response of anaerobic digestion
title_fullStr Autoinducer-2-mediated quorum sensing partially regulates the toxic shock response of anaerobic digestion
title_full_unstemmed Autoinducer-2-mediated quorum sensing partially regulates the toxic shock response of anaerobic digestion
title_sort autoinducer-2-mediated quorum sensing partially regulates the toxic shock response of anaerobic digestion
publishDate 2021
url https://hdl.handle.net/10356/151702
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