Variably improved microbial source tracking with digital droplet PCR

• Main Authors: Nshimyimana, Jean Pierre, Cruz, Mercedes Cecilia, Wuertz, Stefan, Thompson, Janelle R.
• Other Authors: School of Civil and Environmental Engineering
• Format: Article
• Language: English
• Published: 2021
• Subjects: Engineering::Environmental engineering; Microbial Source Tracking; Digital Droplet PCR
• Online Access: https://hdl.handle.net/10356/151711
• Institution: Nanyang Technological University

This study addressed whether digital droplet PCR (ddPCR) could improve sensitivity and specificity of human-associated Bacteroidales genetic markers, BacHum and B. theta, and their quantification in environmental and fecal composite samples. Human markers were quantified by qPCR and ddPCR platforms...