Extracellular polymeric substances of biofilms : suffering from an identity crisis
Microbial biofilms can be both cause and cure to a range of emerging societal problems including antimicrobial tolerance, water sanitation, water scarcity and pollution. The identities of extracellular polymeric substances (EPS) responsible for the establishment and function of biofilms are poorly u...
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sg-ntu-dr.10356-1517162021-06-24T08:13:04Z Extracellular polymeric substances of biofilms : suffering from an identity crisis Seviour, Thomas Derlon, Nicolas Dueholm, Morten Simonsen Flemming, Hans-Curt Girbal-Neuhauser, Elisabeth Horn, Harald Kjelleberg, Staffan van Loosdrecht, Mark C. M. Lotti, Tommaso Malpei, M. Francesca Nerenberg, Robert Neu, Thomas R. Paul, Etienne Yu, Hanqing Lin, Yuemei Singapore Centre for Environmental Life Sciences and Engineering Engineering::Environmental engineering Biofilms Extracellular Polymeric Substances Microbial biofilms can be both cause and cure to a range of emerging societal problems including antimicrobial tolerance, water sanitation, water scarcity and pollution. The identities of extracellular polymeric substances (EPS) responsible for the establishment and function of biofilms are poorly understood. The lack of information on the chemical and physical identities of EPS limits the potential to rationally engineer biofilm processes, and impedes progress within the water and wastewater sector towards a circular economy and resource recovery. Here, a multidisciplinary roadmap for addressing this EPS identity crisis is proposed. This involves improved EPS extraction and characterization methodologies, cross-referencing between model biofilms and full-scale biofilm systems, and functional description of isolated EPS with in situ techniques (e.g. microscopy) coupled with genomics, proteomics and glycomics. The current extraction and spectrophotometric characterization methods, often based on the principle not to compromise the integrity of the microbial cells, should be critically assessed, and more comprehensive methods for recovery and characterization of EPS need to be developed. National Research Foundation (NRF) The collaboration was supported by Singapore National Research Foundation and Ministry of Education under the Research Centre of Excellence Programme, by a program grant from the National Research Foundation (NRF), project number 1301-IRIS-59 (TS); by the SIAM Gravitation 024.002.002, the Netherlands Organisation for Scientific Research and KNAW 530-6CDP15, Koninklijke Nederlandse Akademie van Wetenschappen (YL); by the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie Individual Fellowship grant agreement No 661429 (TL); by the US National Science Foundation award CBET 1605177 (RN). 2021-06-24T08:13:04Z 2021-06-24T08:13:04Z 2018 Journal Article Seviour, T., Derlon, N., Dueholm, M. S., Flemming, H., Girbal-Neuhauser, E., Horn, H., Kjelleberg, S., van Loosdrecht, M. C. M., Lotti, T., Malpei, M. F., Nerenberg, R., Neu, T. R., Paul, E., Yu, H. & Lin, Y. (2018). Extracellular polymeric substances of biofilms : suffering from an identity crisis. Water Research, 151, 1-7. https://dx.doi.org/10.1016/j.watres.2018.11.020 0043-1354 0000-0003-4240-9827 0000-0003-4135-2670 0000-0003-2203-5004 https://hdl.handle.net/10356/151716 10.1016/j.watres.2018.11.020 30557778 2-s2.0-85058223785 151 1 7 en 1301-IRIS-59 Water Research © 2018 Elsevier Ltd. All rights reserved. |
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Engineering::Environmental engineering Biofilms Extracellular Polymeric Substances Seviour, Thomas Derlon, Nicolas Dueholm, Morten Simonsen Flemming, Hans-Curt Girbal-Neuhauser, Elisabeth Horn, Harald Kjelleberg, Staffan van Loosdrecht, Mark C. M. Lotti, Tommaso Malpei, M. Francesca Nerenberg, Robert Neu, Thomas R. Paul, Etienne Yu, Hanqing Lin, Yuemei Extracellular polymeric substances of biofilms : suffering from an identity crisis |
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Microbial biofilms can be both cause and cure to a range of emerging societal problems including antimicrobial tolerance, water sanitation, water scarcity and pollution. The identities of extracellular polymeric substances (EPS) responsible for the establishment and function of biofilms are poorly understood. The lack of information on the chemical and physical identities of EPS limits the potential to rationally engineer biofilm processes, and impedes progress within the water and wastewater sector towards a circular economy and resource recovery. Here, a multidisciplinary roadmap for addressing this EPS identity crisis is proposed. This involves improved EPS extraction and characterization methodologies, cross-referencing between model biofilms and full-scale biofilm systems, and functional description of isolated EPS with in situ techniques (e.g. microscopy) coupled with genomics, proteomics and glycomics. The current extraction and spectrophotometric characterization methods, often based on the principle not to compromise the integrity of the microbial cells, should be critically assessed, and more comprehensive methods for recovery and characterization of EPS need to be developed. |
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Singapore Centre for Environmental Life Sciences and Engineering |
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Singapore Centre for Environmental Life Sciences and Engineering Seviour, Thomas Derlon, Nicolas Dueholm, Morten Simonsen Flemming, Hans-Curt Girbal-Neuhauser, Elisabeth Horn, Harald Kjelleberg, Staffan van Loosdrecht, Mark C. M. Lotti, Tommaso Malpei, M. Francesca Nerenberg, Robert Neu, Thomas R. Paul, Etienne Yu, Hanqing Lin, Yuemei |
format |
Article |
author |
Seviour, Thomas Derlon, Nicolas Dueholm, Morten Simonsen Flemming, Hans-Curt Girbal-Neuhauser, Elisabeth Horn, Harald Kjelleberg, Staffan van Loosdrecht, Mark C. M. Lotti, Tommaso Malpei, M. Francesca Nerenberg, Robert Neu, Thomas R. Paul, Etienne Yu, Hanqing Lin, Yuemei |
author_sort |
Seviour, Thomas |
title |
Extracellular polymeric substances of biofilms : suffering from an identity crisis |
title_short |
Extracellular polymeric substances of biofilms : suffering from an identity crisis |
title_full |
Extracellular polymeric substances of biofilms : suffering from an identity crisis |
title_fullStr |
Extracellular polymeric substances of biofilms : suffering from an identity crisis |
title_full_unstemmed |
Extracellular polymeric substances of biofilms : suffering from an identity crisis |
title_sort |
extracellular polymeric substances of biofilms : suffering from an identity crisis |
publishDate |
2021 |
url |
https://hdl.handle.net/10356/151716 |
_version_ |
1703971258974076928 |