Photophysics of DFHBI bound to RNA aptamer baby spinach

• Main Authors: Dao, Nguyen Thuan, Haselsberger, Reinhard, Khuc, Mai-Thu, Phan, Anh Tuân, Voityuk, Alexander A., Michel-Beyerle, Maria-Elisabeth
• Other Authors: School of Physical and Mathematical Sciences
• Format: Article
• Language: English
• Published: 2021
• Subjects: Science::Biological sciences; Chemical Biology; Molecular Biology
• Online Access: https://hdl.handle.net/10356/151981
• Institution: Nanyang Technological University

The discovery of the GFP-type dye DFHBI that becomes fluorescent upon binding to an RNA aptamer, termed Spinach, led to the development of a variety of fluorogenic RNA systems that enable genetic encoding of living cells. In view of increasing interest in small RNA aptamers and the scarcity of their...