Removing auto-activators from yeast-two-hybrid assays by conditional negative selection

• Main Authors: Shivhare, Devendra, Musialak-Lange, Magdalena, Julca, Irene, Gluza, Pawel, Mutwil, Marek
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2021
• Subjects: Science::Biological sciences; High-Throughput Screening; Protein–Protein Interaction Networks
• Online Access: https://hdl.handle.net/10356/151992
• Institution: Nanyang Technological University

Yeast-two-hybrid (Y2H) is widely used as a strategy to detect protein–protein interactions (PPIs). Recent advancements have made it possible to generate and analyse genome-wide PPI networks en masse by coupling Y2H with next-generation sequencing technology. However, one of the major challenges of y...