Optimization and fabrication of electrospun PCL poly (ε-caprolactone) fiber diameter and 3D scaffold for tissue engineering

Electrospinning of scaffolds using a (biocompatible) polymer solution for tissue engineering is an approach that is gaining popularity. In order to increase the potential that a scaffold can bring in the biomedical field, reduction of fiber diameters and optimization of scaffold porosity through...

Full description

Saved in:
Bibliographic Details
Main Author: Lim, Marcus Zhi Wei.
Other Authors: Tan Lay Poh
Format: Final Year Project
Language:English
Published: 2009
Subjects:
Online Access:http://hdl.handle.net/10356/15315
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Nanyang Technological University
Language: English
id sg-ntu-dr.10356-15315
record_format dspace
spelling sg-ntu-dr.10356-153152023-03-04T15:38:41Z Optimization and fabrication of electrospun PCL poly (ε-caprolactone) fiber diameter and 3D scaffold for tissue engineering Lim, Marcus Zhi Wei. Tan Lay Poh School of Materials Science and Engineering DRNTU::Engineering::Materials::Biomaterials DRNTU::Science::Medicine::Tissue engineering DRNTU::Engineering::Materials::Nanostructured materials DRNTU::Science::Medicine::Biomedical engineering Electrospinning of scaffolds using a (biocompatible) polymer solution for tissue engineering is an approach that is gaining popularity. In order to increase the potential that a scaffold can bring in the biomedical field, reduction of fiber diameters and optimization of scaffold porosity through the fabrication of a three-dimensional (3D) scaffold to improve on the capabilities of existing two-dimensional (2D) scaffolds that has to be carried out. In this study, Poly ( -caprolactone) was the material used for the electrospun scaffold. The study was divided into three parts. Firstly, manipulation of various polymer solution and electrospinning machine chamber parameters to optimize fiber diameters was performed. Once optimum fiber diameters was obtained, a novel method of electrospinning on a conductive, needle-like target was done to produce spherical 3D scaffolds. At the same time, electrospinning of fibers was also conducted on PCL Rapid Prototype Scaffolds (RPS) acting as a framework for production of 3D scaffolds. The optimum solution mixture obtained consisted of Dichloromethane (DCM) for dissolving PCL and N,N-Dimethylforamide (DMF) as an additive to increase solution conductivity in the ratio of 3.5:6.5. Machine parameters were set at a voltage of 18kV and a feed rate of 0.5ml/h. The 3D scaffolds electrospun using both methods using these parameters were subjected to morphological characterization. The 3D scaffolds showed obvious signs of porosity and tests show that the level of penetration was more than 90%. Unfortunately, the spinning of 3D scaffolds with a PCL RPS did not yield a 3D nonwoven PCL structure. While the project was able to lead to optimization of fiber parameters and show that scaffolds have a relatively high level of porosity, the utilization of the RPS can be developed further. Despite these limitations, the potential benefits a 3D electrospun scaffold can bring makes it a promising area worthy of further improvement. Future studies could be engineered towards the direction of improvement of the RPS electrospinning techniques to build 3D scaffolds in alternate ways. Bachelor of Engineering (Materials Engineering) 2009-04-27T07:36:21Z 2009-04-27T07:36:21Z 2009 2009 Final Year Project (FYP) http://hdl.handle.net/10356/15315 en Nanyang Technological University 63 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Materials::Biomaterials
DRNTU::Science::Medicine::Tissue engineering
DRNTU::Engineering::Materials::Nanostructured materials
DRNTU::Science::Medicine::Biomedical engineering
spellingShingle DRNTU::Engineering::Materials::Biomaterials
DRNTU::Science::Medicine::Tissue engineering
DRNTU::Engineering::Materials::Nanostructured materials
DRNTU::Science::Medicine::Biomedical engineering
Lim, Marcus Zhi Wei.
Optimization and fabrication of electrospun PCL poly (ε-caprolactone) fiber diameter and 3D scaffold for tissue engineering
description Electrospinning of scaffolds using a (biocompatible) polymer solution for tissue engineering is an approach that is gaining popularity. In order to increase the potential that a scaffold can bring in the biomedical field, reduction of fiber diameters and optimization of scaffold porosity through the fabrication of a three-dimensional (3D) scaffold to improve on the capabilities of existing two-dimensional (2D) scaffolds that has to be carried out. In this study, Poly ( -caprolactone) was the material used for the electrospun scaffold. The study was divided into three parts. Firstly, manipulation of various polymer solution and electrospinning machine chamber parameters to optimize fiber diameters was performed. Once optimum fiber diameters was obtained, a novel method of electrospinning on a conductive, needle-like target was done to produce spherical 3D scaffolds. At the same time, electrospinning of fibers was also conducted on PCL Rapid Prototype Scaffolds (RPS) acting as a framework for production of 3D scaffolds. The optimum solution mixture obtained consisted of Dichloromethane (DCM) for dissolving PCL and N,N-Dimethylforamide (DMF) as an additive to increase solution conductivity in the ratio of 3.5:6.5. Machine parameters were set at a voltage of 18kV and a feed rate of 0.5ml/h. The 3D scaffolds electrospun using both methods using these parameters were subjected to morphological characterization. The 3D scaffolds showed obvious signs of porosity and tests show that the level of penetration was more than 90%. Unfortunately, the spinning of 3D scaffolds with a PCL RPS did not yield a 3D nonwoven PCL structure. While the project was able to lead to optimization of fiber parameters and show that scaffolds have a relatively high level of porosity, the utilization of the RPS can be developed further. Despite these limitations, the potential benefits a 3D electrospun scaffold can bring makes it a promising area worthy of further improvement. Future studies could be engineered towards the direction of improvement of the RPS electrospinning techniques to build 3D scaffolds in alternate ways.
author2 Tan Lay Poh
author_facet Tan Lay Poh
Lim, Marcus Zhi Wei.
format Final Year Project
author Lim, Marcus Zhi Wei.
author_sort Lim, Marcus Zhi Wei.
title Optimization and fabrication of electrospun PCL poly (ε-caprolactone) fiber diameter and 3D scaffold for tissue engineering
title_short Optimization and fabrication of electrospun PCL poly (ε-caprolactone) fiber diameter and 3D scaffold for tissue engineering
title_full Optimization and fabrication of electrospun PCL poly (ε-caprolactone) fiber diameter and 3D scaffold for tissue engineering
title_fullStr Optimization and fabrication of electrospun PCL poly (ε-caprolactone) fiber diameter and 3D scaffold for tissue engineering
title_full_unstemmed Optimization and fabrication of electrospun PCL poly (ε-caprolactone) fiber diameter and 3D scaffold for tissue engineering
title_sort optimization and fabrication of electrospun pcl poly (ε-caprolactone) fiber diameter and 3d scaffold for tissue engineering
publishDate 2009
url http://hdl.handle.net/10356/15315
_version_ 1759857447060635648