In vitro protein (BSA) delivery studies in mesostructured bioceramics

• Main Author: Aw, Siew Sim
• Other Authors: Loo Say Chye Joachim
• Format: Final Year Project
• Language: English
• Published: 2009
• Subjects: DRNTU::Engineering::Materials::Biomaterials
• Online Access: http://hdl.handle.net/10356/15344
• Institution: Nanyang Technological University

3D mesoporous bioactive glasses (MBGs), SiO2-CaO-P2O5, with four different chemical compositions and three different synthesis temperatures during synthesis had been successfully prepared by F127 surfactant as templates using evaporation-induced self-assembly (EISA) process. Mesoporous calcium hydroxyapatite (MHA), Ca10(PO4)6(OH)2, was prepared for comparison as well. From this project, to better control of structural parameter of final synthesized sample varying the silica composition content and synthesis temperature are being carried out. Synthesis samples with higher silica composition content produce higher surface area but lower pore volume and pore size. Higher silica composition content of 80S and 90S produce a better result of synthesize at room temperature. Both mesostructured bioceramics used in protein delivery systems using Bovine Serum Albumin (BSA) as protein model. The influence of matrix parameters in surface area, pore volume and pore size in synthesized samples and with controlled delivery were revised. All synthesized MBG and MHA have the mesoporous porous system (pore diameters of between 2 and 50nm). BSA was then being encapsulated into few selected synthesized MBG and MHA by adsorption and releasing method was performed. The loading and release result shown that MBG has a better protein delivery system than MHA. Its samples before and after BSA loadings were characterized using nitrogen adsorption-desorption, TG analyses, X-ray diffraction, FT-IR spectroscopy and UV spectrometer. The results in the report show that pore size is generally the dominant structural parameter for controlling the structural parameters and protein delivery in the samples.