Anticancer potential of L-histidine-capped silver nanoparticles against human cervical cancer cells (SIHA)

• Main Authors: Mohammed Asik, Rajmohamed, Manikkaraja, Chidhambaram, Tamil Surya, Karuppusamy, Suganthy, Natarajan, Priya Aarthy, Archunan, Mathe, Domokos, Sivakumar, Muthusamy, Archunan, Govindaraju, Padmanabhan, Parasuraman, Gulyás, Balázs
• Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
• Format: Article
• Language: English
• Published: 2021
• Subjects: Science::Medicine; Anticancer Activity; L-Histidine
• Online Access: https://hdl.handle.net/10356/153510
• Institution: Nanyang Technological University

This study reports the synthesis of silver nanoparticles using amino acid L-histidine as a reducing and capping agent as an eco-friendly approach. Fabricated L-histidine-capped silver nanoparticles (L-HAgNPs) were characterized by spectroscopic and microscopic studies. Spherical shaped L-HAgNPs were synthesized with a particle size of 47.43 ± 19.83 nm and zeta potential of -20.5 ± 0.95 mV. Results of the anticancer potential of L-HAgNPs showed antiproliferative effect against SiHa cells in a dose-dependent manner with an IC50 value of 18.25 ± 0.36 µg/mL. Fluorescent microscopic analysis revealed L-HAgNPs induced reactive oxygen species (ROS) mediated mitochondrial dysfunction, leading to activation of apoptotic pathway and DNA damage eventually causing cell death. To conclude, L-HAgNPs can act as promising candidates for cervical cancer therapy.