A rapid CRISPR competitive assay for in vitro and in vivo discovery of potential drug targets affecting the hematopoietic system

• Main Authors: Shen, Yunbing, Jiang, Long, Iyer, Vaishnavi Srinivasan, Raposo, Bruno, Dubnovitsky, Anatoly, Boddul, Sanjaykumar V., Kasza, Zsolt, Wermeling, Fredrik
• Other Authors: School of Physical and Mathematical Sciences
• Format: Article
• Language: English
• Published: 2021
• Subjects: Science::Medicine; Science::Biological sciences; Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR); Sequence Analysis
• Online Access: https://hdl.handle.net/10356/153758
• Institution: Nanyang Technological University

CRISPR/Cas9 can be used as an experimental tool to inactivate genes in cells. However, a CRISPR-targeted cell population will not show a uniform genotype of the targeted gene. Instead, a mix of genotypes is generated - from wild type to different forms of insertions and deletions. Such mixed genotyp...