Ocular delivery of predatory bacteria with cryomicroneedles against eye infection
The development of potent antibiotic alternatives with rapid bactericidal properties is of great importance in addressing the current antibiotic crisis. One representative example is the topical delivery of predatory bacteria to treat ocular bacterial infections. However, there is a lack of suitable...
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sg-ntu-dr.10356-1538062023-12-29T06:53:48Z Ocular delivery of predatory bacteria with cryomicroneedles against eye infection Cui, Mingyue Zheng, Mengjia Wiraja, Christian Chew, Sharon Wan Ting Mishra, Arti Mayandi, Venkatesh Lakshminarayanan, Rajamani Xu, Chenjie School of Chemical and Biomedical Engineering Engineering::Chemical engineering Cryomicroneedles Drug Delivery The development of potent antibiotic alternatives with rapid bactericidal properties is of great importance in addressing the current antibiotic crisis. One representative example is the topical delivery of predatory bacteria to treat ocular bacterial infections. However, there is a lack of suitable methods for the delivery of predatory bacteria into ocular tissue. This work introduces cryomicroneedles (cryoMN) for the ocular delivery of predatory Bdellovibrio bacteriovorus (B. bacteriovorus) bacteria. The cryoMN patches are prepared by freezing B. bacteriovorus containing a cryoprotectant medium in a microneedle template. The viability of B. bacteriovorus in cryoMNs remains above 80% as found in long-term storage studies, and they successfully impede the growth of gram-negative bacteria in vitro or in a rodent eye infection model. The infection is significantly relieved by nearly six times through 2.5 days of treatment without substantial effects on the cornea thickness and morphology. This approach represents the safe and efficient delivery of new class of antimicrobial armamentarium to otherwise impermeable ocular surface and opens up new avenues for the treatment of ocular surface disorders. Ministry of Health (MOH) National Medical Research Council (NMRC) Published version C.X. acknowledges the funding support from City University of Hong Kong (#9610472), General Research Fund (GRF) from University Grant Committee of Hong Kong (UGC) Research Grant Council (RGC) (#9042951 and #9043133), and NSFC/RGC Joint Research Scheme (N_CityU118/20#9054032). R.L. thanks funding support from the Singapore Ministry of Health’s National Medical Research Council under its Centre Grant Programme – Optimization of Core Platform Technologies for Ocular Research-NMRC/CG/M010/2017_SERI and the SingHealth Foundation (SHF/FG663P/2017). The authors thank Dr. VA Barathi for her help with the animal facilities at SERI. 2021-12-14T06:59:14Z 2021-12-14T06:59:14Z 2021 Journal Article Cui, M., Zheng, M., Wiraja, C., Chew, S. W. T., Mishra, A., Mayandi, V., Lakshminarayanan, R. & Xu, C. (2021). Ocular delivery of predatory bacteria with cryomicroneedles against eye infection. Advanced Science, 8(21), 2102327-. https://dx.doi.org/10.1002/advs.202102327 2198-3844 https://hdl.handle.net/10356/153806 10.1002/advs.202102327 34494724 2-s2.0-85114357126 21 8 2102327 en SHF/FG663P/2017 NMRC/CG/M010/2017_SER Advanced Science © 2021 The Authors. Advanced Science published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. application/pdf |
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Engineering::Chemical engineering Cryomicroneedles Drug Delivery Cui, Mingyue Zheng, Mengjia Wiraja, Christian Chew, Sharon Wan Ting Mishra, Arti Mayandi, Venkatesh Lakshminarayanan, Rajamani Xu, Chenjie Ocular delivery of predatory bacteria with cryomicroneedles against eye infection |
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The development of potent antibiotic alternatives with rapid bactericidal properties is of great importance in addressing the current antibiotic crisis. One representative example is the topical delivery of predatory bacteria to treat ocular bacterial infections. However, there is a lack of suitable methods for the delivery of predatory bacteria into ocular tissue. This work introduces cryomicroneedles (cryoMN) for the ocular delivery of predatory Bdellovibrio bacteriovorus (B. bacteriovorus) bacteria. The cryoMN patches are prepared by freezing B. bacteriovorus containing a cryoprotectant medium in a microneedle template. The viability of B. bacteriovorus in cryoMNs remains above 80% as found in long-term storage studies, and they successfully impede the growth of gram-negative bacteria in vitro or in a rodent eye infection model. The infection is significantly relieved by nearly six times through 2.5 days of treatment without substantial effects on the cornea thickness and morphology. This approach represents the safe and efficient delivery of new class of antimicrobial armamentarium to otherwise impermeable ocular surface and opens up new avenues for the treatment of ocular surface disorders. |
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School of Chemical and Biomedical Engineering |
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School of Chemical and Biomedical Engineering Cui, Mingyue Zheng, Mengjia Wiraja, Christian Chew, Sharon Wan Ting Mishra, Arti Mayandi, Venkatesh Lakshminarayanan, Rajamani Xu, Chenjie |
format |
Article |
author |
Cui, Mingyue Zheng, Mengjia Wiraja, Christian Chew, Sharon Wan Ting Mishra, Arti Mayandi, Venkatesh Lakshminarayanan, Rajamani Xu, Chenjie |
author_sort |
Cui, Mingyue |
title |
Ocular delivery of predatory bacteria with cryomicroneedles against eye infection |
title_short |
Ocular delivery of predatory bacteria with cryomicroneedles against eye infection |
title_full |
Ocular delivery of predatory bacteria with cryomicroneedles against eye infection |
title_fullStr |
Ocular delivery of predatory bacteria with cryomicroneedles against eye infection |
title_full_unstemmed |
Ocular delivery of predatory bacteria with cryomicroneedles against eye infection |
title_sort |
ocular delivery of predatory bacteria with cryomicroneedles against eye infection |
publishDate |
2021 |
url |
https://hdl.handle.net/10356/153806 |
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1787136804183343104 |