Formin nanoclustering-mediated actin assembly during plant flagellin and DSF signaling

Plants respond to bacterial infection acutely with actin remodeling during plant immune responses. The mechanisms by which bacterial virulence factors (VFs) modulate plant actin polymerization remain enigmatic. Here, we show that plant-type-I formin serves as the molecular sensor for actin remodelin...

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Main Authors: Ma, Zhiming, Liu, Xiaolin, Nath, Sangeeta, Sun, He, Tran, Tuan Minh, Yang, Liang, Mayor, Satyajit, Miao, Yansong
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2022
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Online Access:https://hdl.handle.net/10356/153816
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-1538162022-02-19T20:11:42Z Formin nanoclustering-mediated actin assembly during plant flagellin and DSF signaling Ma, Zhiming Liu, Xiaolin Nath, Sangeeta Sun, He Tran, Tuan Minh Yang, Liang Mayor, Satyajit Miao, Yansong School of Biological Sciences Singapore Centre for Environmental Life Sciences and Engineering (SCELSE) Science::Biological sciences Actin Remodeling Actin Nucleation Plants respond to bacterial infection acutely with actin remodeling during plant immune responses. The mechanisms by which bacterial virulence factors (VFs) modulate plant actin polymerization remain enigmatic. Here, we show that plant-type-I formin serves as the molecular sensor for actin remodeling in response to two bacterial VFs: Xanthomonas campestris pv. campestris (Xcc) diffusible signal factor (DSF), and pathogen-associated molecular pattern (PAMP) flagellin in pattern-triggered immunity (PTI). Both VFs regulate actin assembly by tuning the clustering and nucleation activity of formin on the plasma membrane (PM) at the nano-sized scale. By being integrated within the cell-wall-PM-actin cytoskeleton (CW-PM-AC) continuum, the dynamic behavior and function of formins are highly dependent on each scaffold layer's composition within the CW-PM-AC continuum during both DSF and PTI signaling. Our results reveal a central mechanism for rapid actin remodeling during plant-bacteria interactions, in which bacterial signaling molecules fine tune plant formin nanoclustering in a host mechanical-structure-dependent manner. Ministry of Education (MOE) Nanyang Technological University Published version S.M. is supported by a Margdarshi Fellowship (IA/M/15/1/502018) from the DBT-Wellcome Trust Alliance and the Department of Atomic Energy (Government of India) under project no. RTI 4006 to National Centre for Biological Sciences, India. This study was supported by an NTU startup grant (M4081533) and the Singapore Ministry of Education (MOE) tiers 1 (RG32/20) and 3 (MOE2019-T3-1-012) to Y.M. 2022-02-15T08:32:11Z 2022-02-15T08:32:11Z 2021 Journal Article Ma, Z., Liu, X., Nath, S., Sun, H., Tran, T. M., Yang, L., Mayor, S. & Miao, Y. (2021). Formin nanoclustering-mediated actin assembly during plant flagellin and DSF signaling. Cell Reports, 34(13), 108884-. https://dx.doi.org/10.1016/j.celrep.2021.108884 2211-1247 https://hdl.handle.net/10356/153816 10.1016/j.celrep.2021.108884 33789103 2-s2.0-85103419548 13 34 108884 en M4081533 RG32/20 MOE2019-T3-1-012 Cell Reports © 2021 The Author(s). This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Biological sciences
Actin Remodeling
Actin Nucleation
spellingShingle Science::Biological sciences
Actin Remodeling
Actin Nucleation
Ma, Zhiming
Liu, Xiaolin
Nath, Sangeeta
Sun, He
Tran, Tuan Minh
Yang, Liang
Mayor, Satyajit
Miao, Yansong
Formin nanoclustering-mediated actin assembly during plant flagellin and DSF signaling
description Plants respond to bacterial infection acutely with actin remodeling during plant immune responses. The mechanisms by which bacterial virulence factors (VFs) modulate plant actin polymerization remain enigmatic. Here, we show that plant-type-I formin serves as the molecular sensor for actin remodeling in response to two bacterial VFs: Xanthomonas campestris pv. campestris (Xcc) diffusible signal factor (DSF), and pathogen-associated molecular pattern (PAMP) flagellin in pattern-triggered immunity (PTI). Both VFs regulate actin assembly by tuning the clustering and nucleation activity of formin on the plasma membrane (PM) at the nano-sized scale. By being integrated within the cell-wall-PM-actin cytoskeleton (CW-PM-AC) continuum, the dynamic behavior and function of formins are highly dependent on each scaffold layer's composition within the CW-PM-AC continuum during both DSF and PTI signaling. Our results reveal a central mechanism for rapid actin remodeling during plant-bacteria interactions, in which bacterial signaling molecules fine tune plant formin nanoclustering in a host mechanical-structure-dependent manner.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Ma, Zhiming
Liu, Xiaolin
Nath, Sangeeta
Sun, He
Tran, Tuan Minh
Yang, Liang
Mayor, Satyajit
Miao, Yansong
format Article
author Ma, Zhiming
Liu, Xiaolin
Nath, Sangeeta
Sun, He
Tran, Tuan Minh
Yang, Liang
Mayor, Satyajit
Miao, Yansong
author_sort Ma, Zhiming
title Formin nanoclustering-mediated actin assembly during plant flagellin and DSF signaling
title_short Formin nanoclustering-mediated actin assembly during plant flagellin and DSF signaling
title_full Formin nanoclustering-mediated actin assembly during plant flagellin and DSF signaling
title_fullStr Formin nanoclustering-mediated actin assembly during plant flagellin and DSF signaling
title_full_unstemmed Formin nanoclustering-mediated actin assembly during plant flagellin and DSF signaling
title_sort formin nanoclustering-mediated actin assembly during plant flagellin and dsf signaling
publishDate 2022
url https://hdl.handle.net/10356/153816
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