Silver nanoprism-based plasmonic ELISA for sensitive detection of fluoroquinolones
Fluoroquinolones are synthetic antibiotics that are commonly used in animal husbandry, and the consumption of animal products with fluoroquinolone residues has imposed a serious threat to human health. Here, we report a plasmonic enzyme-linked immunosorbent assay (pELISA) method based on oxidative e...
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sg-ntu-dr.10356-1542342021-12-16T04:29:14Z Silver nanoprism-based plasmonic ELISA for sensitive detection of fluoroquinolones Yuan, Meifang Xiong, Qirong Zhang, Ganggang Xiong, Zhijuan Liu, Daofeng Duan, Hongwei Lai, Weihua School of Chemical and Biomedical Engineering Engineering::Chemical engineering Animals Antibiotics Fluoroquinolones are synthetic antibiotics that are commonly used in animal husbandry, and the consumption of animal products with fluoroquinolone residues has imposed a serious threat to human health. Here, we report a plasmonic enzyme-linked immunosorbent assay (pELISA) method based on oxidative etching of silver nanoprisms (AgNPRs) for the quantitative and qualitative detection of danofloxacin (DAN), a fluoroquinolone antibiotic. AgNPRs that undergo colorimetric changes upon oxidative etching by H2O2 serve as the signal transducer in our design. An indirect competitive pELISA was constructed by introducing biotinylated monoclonal antibody (mAb), streptavidin and biotinylated glucose oxidase, which catalyzes the generation of H2O2 for etching AgNPRs. The quantitative detection limit of the proposed method was 0.24 ng mL-1 for DAN. The qualitative detection limit for DAN reached 0.32 ng mL-1, which was 32-fold lower than that of the assay using 3,3',5,5'-tetramethylbenzidine (TMB) as the signal transducer. The average recoveries of DAN in milk ranged from 103% to 121%, with a coefficient of variation of 0.6-3.41%. The recovery results were further confirmed using liquid chromatography-tandem mass spectrometry. In summary, the proposed AgNPR-etching pELISA exhibits high sensitivity, good accuracy and excellent reliability for the quantitative and qualitative detection of DAN in milk. This work was supported by the Program of State Key Laboratory of Food Science and Technology, Nanchang University (SKLF-ZZA-201912), the free explore issue of State Key Laboratory of Food Science and Technology of Nanchang University (SKLF-ZZB-201913), Jiangxi Science Foundation for Young Scientists (20181BAB214018) and the Singapore-MIT Alliance of Research and Technology Center (ING000383-ENG). 2021-12-16T04:29:13Z 2021-12-16T04:29:13Z 2020 Journal Article Yuan, M., Xiong, Q., Zhang, G., Xiong, Z., Liu, D., Duan, H. & Lai, W. (2020). Silver nanoprism-based plasmonic ELISA for sensitive detection of fluoroquinolones. Journal of Materials Chemistry B, 8(16), 3667-3675. https://dx.doi.org/10.1039/c9tb02776a 2050-750X https://hdl.handle.net/10356/154234 10.1039/c9tb02776a 32039414 2-s2.0-85084176840 16 8 3667 3675 en ING000383-ENG Journal of Materials Chemistry B © 2020 The Royal Society of Chemistry. All rights reserved. |
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Engineering::Chemical engineering Animals Antibiotics Yuan, Meifang Xiong, Qirong Zhang, Ganggang Xiong, Zhijuan Liu, Daofeng Duan, Hongwei Lai, Weihua Silver nanoprism-based plasmonic ELISA for sensitive detection of fluoroquinolones |
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Fluoroquinolones are synthetic antibiotics that are commonly used in animal husbandry, and the consumption of animal products with fluoroquinolone residues has imposed a serious threat to human health. Here, we report a plasmonic enzyme-linked immunosorbent assay (pELISA) method based on oxidative etching of silver nanoprisms (AgNPRs) for the quantitative and qualitative detection of danofloxacin (DAN), a fluoroquinolone antibiotic. AgNPRs that undergo colorimetric changes upon oxidative etching by H2O2 serve as the signal transducer in our design. An indirect competitive pELISA was constructed by introducing biotinylated monoclonal antibody (mAb), streptavidin and biotinylated glucose oxidase, which catalyzes the generation of H2O2 for etching AgNPRs. The quantitative detection limit of the proposed method was 0.24 ng mL-1 for DAN. The qualitative detection limit for DAN reached 0.32 ng mL-1, which was 32-fold lower than that of the assay using 3,3',5,5'-tetramethylbenzidine (TMB) as the signal transducer. The average recoveries of DAN in milk ranged from 103% to 121%, with a coefficient of variation of 0.6-3.41%. The recovery results were further confirmed using liquid chromatography-tandem mass spectrometry. In summary, the proposed AgNPR-etching pELISA exhibits high sensitivity, good accuracy and excellent reliability for the quantitative and qualitative detection of DAN in milk. |
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School of Chemical and Biomedical Engineering |
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School of Chemical and Biomedical Engineering Yuan, Meifang Xiong, Qirong Zhang, Ganggang Xiong, Zhijuan Liu, Daofeng Duan, Hongwei Lai, Weihua |
format |
Article |
author |
Yuan, Meifang Xiong, Qirong Zhang, Ganggang Xiong, Zhijuan Liu, Daofeng Duan, Hongwei Lai, Weihua |
author_sort |
Yuan, Meifang |
title |
Silver nanoprism-based plasmonic ELISA for sensitive detection of fluoroquinolones |
title_short |
Silver nanoprism-based plasmonic ELISA for sensitive detection of fluoroquinolones |
title_full |
Silver nanoprism-based plasmonic ELISA for sensitive detection of fluoroquinolones |
title_fullStr |
Silver nanoprism-based plasmonic ELISA for sensitive detection of fluoroquinolones |
title_full_unstemmed |
Silver nanoprism-based plasmonic ELISA for sensitive detection of fluoroquinolones |
title_sort |
silver nanoprism-based plasmonic elisa for sensitive detection of fluoroquinolones |
publishDate |
2021 |
url |
https://hdl.handle.net/10356/154234 |
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1720447198002413568 |