Simultaneous extraction, separation, isolation and identification of endogenous components from Etlingera elatior by pressurized matrix solid-phase dispersion using liquid chromatography-mass spectrometry

From the analytical chemistry point-of-view, an ideal sample preparation method should be simple, rapid, automatic, selective, precise, exhaustive, reproducible and protect the analyte-of-interest from degradation. In this study, a novel sample preparation method, named pressurized matrix solid-phas...

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Main Authors: Ge, Liya, Yong, Jean Wan Hong, Tan, Swee Ngin, Li, Shao-Ping
Other Authors: Nanyang Environment and Water Research Institute
Format: Article
Language:English
Published: 2021
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Online Access:https://hdl.handle.net/10356/154540
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1545402021-12-28T02:34:16Z Simultaneous extraction, separation, isolation and identification of endogenous components from Etlingera elatior by pressurized matrix solid-phase dispersion using liquid chromatography-mass spectrometry Ge, Liya Yong, Jean Wan Hong Tan, Swee Ngin Li, Shao-Ping Nanyang Environment and Water Research Institute Engineering::Environmental engineering Etlingera Elatior Pressurized Matrix Solid-Phase Dispersion From the analytical chemistry point-of-view, an ideal sample preparation method should be simple, rapid, automatic, selective, precise, exhaustive, reproducible and protect the analyte-of-interest from degradation. In this study, a novel sample preparation method, named pressurized matrix solid-phase dispersion (p-MSPD) extraction was developed for simultaneously extracting, separating, purifying, isolating, and analyzing endogenous components in a solid sample matrix. Etlingera elatior, a traditional medicinal plant known as the torch ginger, was applied as a sample matrix to evaluate the p-MSPD process. The entire extraction, separation, isolation, fractionation and detection were performed automatically with a commercial LC-MS system. The novel method was satisfactorily applied for the preparation of real samples without optimization, which had the ability to selectively isolate pure compounds from the solid sample matrix for further NMR analysis. Therefore, the method is recommended for quality control of traditional medicines, research efforts when sample amounts are limited, and laboratories that have ordinary LC-MS instrumentation. 2021-12-28T02:34:16Z 2021-12-28T02:34:16Z 2020 Journal Article Ge, L., Yong, J. W. H., Tan, S. N. & Li, S. (2020). Simultaneous extraction, separation, isolation and identification of endogenous components from Etlingera elatior by pressurized matrix solid-phase dispersion using liquid chromatography-mass spectrometry. Journal of Chromatography A, 1611, 460604-. https://dx.doi.org/10.1016/j.chroma.2019.460604 0021-9673 https://hdl.handle.net/10356/154540 10.1016/j.chroma.2019.460604 31676090 2-s2.0-85074439249 1611 460604 en Journal of Chromatography A © 2019 Elsevier B.V. All rights reserved.
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Environmental engineering
Etlingera Elatior
Pressurized Matrix Solid-Phase Dispersion
spellingShingle Engineering::Environmental engineering
Etlingera Elatior
Pressurized Matrix Solid-Phase Dispersion
Ge, Liya
Yong, Jean Wan Hong
Tan, Swee Ngin
Li, Shao-Ping
Simultaneous extraction, separation, isolation and identification of endogenous components from Etlingera elatior by pressurized matrix solid-phase dispersion using liquid chromatography-mass spectrometry
description From the analytical chemistry point-of-view, an ideal sample preparation method should be simple, rapid, automatic, selective, precise, exhaustive, reproducible and protect the analyte-of-interest from degradation. In this study, a novel sample preparation method, named pressurized matrix solid-phase dispersion (p-MSPD) extraction was developed for simultaneously extracting, separating, purifying, isolating, and analyzing endogenous components in a solid sample matrix. Etlingera elatior, a traditional medicinal plant known as the torch ginger, was applied as a sample matrix to evaluate the p-MSPD process. The entire extraction, separation, isolation, fractionation and detection were performed automatically with a commercial LC-MS system. The novel method was satisfactorily applied for the preparation of real samples without optimization, which had the ability to selectively isolate pure compounds from the solid sample matrix for further NMR analysis. Therefore, the method is recommended for quality control of traditional medicines, research efforts when sample amounts are limited, and laboratories that have ordinary LC-MS instrumentation.
author2 Nanyang Environment and Water Research Institute
author_facet Nanyang Environment and Water Research Institute
Ge, Liya
Yong, Jean Wan Hong
Tan, Swee Ngin
Li, Shao-Ping
format Article
author Ge, Liya
Yong, Jean Wan Hong
Tan, Swee Ngin
Li, Shao-Ping
author_sort Ge, Liya
title Simultaneous extraction, separation, isolation and identification of endogenous components from Etlingera elatior by pressurized matrix solid-phase dispersion using liquid chromatography-mass spectrometry
title_short Simultaneous extraction, separation, isolation and identification of endogenous components from Etlingera elatior by pressurized matrix solid-phase dispersion using liquid chromatography-mass spectrometry
title_full Simultaneous extraction, separation, isolation and identification of endogenous components from Etlingera elatior by pressurized matrix solid-phase dispersion using liquid chromatography-mass spectrometry
title_fullStr Simultaneous extraction, separation, isolation and identification of endogenous components from Etlingera elatior by pressurized matrix solid-phase dispersion using liquid chromatography-mass spectrometry
title_full_unstemmed Simultaneous extraction, separation, isolation and identification of endogenous components from Etlingera elatior by pressurized matrix solid-phase dispersion using liquid chromatography-mass spectrometry
title_sort simultaneous extraction, separation, isolation and identification of endogenous components from etlingera elatior by pressurized matrix solid-phase dispersion using liquid chromatography-mass spectrometry
publishDate 2021
url https://hdl.handle.net/10356/154540
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