Osteoinductive effects of 1α, 25 Dihydroxyvitamin D3 on human mesenchymal stem cells.

Bone healing poses a challenge for clinicians due to the bone’s poor regenerative property. While autologous bone grafting remains the most common solution for bone defects, it causes various proven side effects. Recent developments seem to suggest that cell-based bone tissue engineering (BTE) invol...

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Main Author: Toh, Tai Chong.
Other Authors: School of Biological Sciences
Format: Final Year Project
Language:English
Published: 2009
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Online Access:http://hdl.handle.net/10356/15457
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-154572023-02-28T18:03:48Z Osteoinductive effects of 1α, 25 Dihydroxyvitamin D3 on human mesenchymal stem cells. Toh, Tai Chong. School of Biological Sciences A*STAR Institute of Bioengineering and Nanotechnology Lou, Yan Ru Yue, Zhilian DRNTU::Science::Biological sciences::Cytology Bone healing poses a challenge for clinicians due to the bone’s poor regenerative property. While autologous bone grafting remains the most common solution for bone defects, it causes various proven side effects. Recent developments seem to suggest that cell-based bone tissue engineering (BTE) involving human mesenchymal stem cells (hMSCs) is a more viable solution. This project aims to characterize the proliferative and osteoinductive effects of 1,25-(OH)2-D3 on hMSCs, by subjecting the cells to various concentrations of 1,25-(OH)2-D3 in vitro. Our results highlighted the dose-dependent growth inhibitory effects on hMSCs after 1,25-(OH)2-D3 administration. In addition, we have shown that supra-physiological concentrations (1 nM and 10 nM) of 1,25-(OH)2-D3 up-regulated various osteogenic markers including alkaline phosphatase (ALP) activity, extracellular mineralization, osteocalcin secretion, mRNA expression of osteocalcin and osteopontin , and also promoted early onset of hMSC morphological changes. Conversely, physiological concentrations (0.05 nM and 0.1 nM) of 1,25-(OH)2-D3 produced limited osteoinductive effects in vitro. As a practical application, 1,25-(OH)2-D3 was encapsulated in a nanoparticle, PEGylated polyaspartic acid with L-phenylalanine side chains (PAph-5), as a vehicle for in vitro delivery to hMSCs. This project has underlined the clinical implications of 1,25-(OH)2-D3 and demonstrated its potential application to BTE with the aid of nanotechnology. *I will like to express my gratitude to Institute of Bioengineering and Nanotechnology (IBN), IBN Youth Research Program, Biomedical Research Council and A*STAR, for their support that made this work possible. Bachelor of Science in Biological Sciences 2009-05-04T04:49:15Z 2009-05-04T04:49:15Z 2009 2009 Final Year Project (FYP) http://hdl.handle.net/10356/15457 en Nanyang Technological University 46 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Cytology
spellingShingle DRNTU::Science::Biological sciences::Cytology
Toh, Tai Chong.
Osteoinductive effects of 1α, 25 Dihydroxyvitamin D3 on human mesenchymal stem cells.
description Bone healing poses a challenge for clinicians due to the bone’s poor regenerative property. While autologous bone grafting remains the most common solution for bone defects, it causes various proven side effects. Recent developments seem to suggest that cell-based bone tissue engineering (BTE) involving human mesenchymal stem cells (hMSCs) is a more viable solution. This project aims to characterize the proliferative and osteoinductive effects of 1,25-(OH)2-D3 on hMSCs, by subjecting the cells to various concentrations of 1,25-(OH)2-D3 in vitro. Our results highlighted the dose-dependent growth inhibitory effects on hMSCs after 1,25-(OH)2-D3 administration. In addition, we have shown that supra-physiological concentrations (1 nM and 10 nM) of 1,25-(OH)2-D3 up-regulated various osteogenic markers including alkaline phosphatase (ALP) activity, extracellular mineralization, osteocalcin secretion, mRNA expression of osteocalcin and osteopontin , and also promoted early onset of hMSC morphological changes. Conversely, physiological concentrations (0.05 nM and 0.1 nM) of 1,25-(OH)2-D3 produced limited osteoinductive effects in vitro. As a practical application, 1,25-(OH)2-D3 was encapsulated in a nanoparticle, PEGylated polyaspartic acid with L-phenylalanine side chains (PAph-5), as a vehicle for in vitro delivery to hMSCs. This project has underlined the clinical implications of 1,25-(OH)2-D3 and demonstrated its potential application to BTE with the aid of nanotechnology. *I will like to express my gratitude to Institute of Bioengineering and Nanotechnology (IBN), IBN Youth Research Program, Biomedical Research Council and A*STAR, for their support that made this work possible.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Toh, Tai Chong.
format Final Year Project
author Toh, Tai Chong.
author_sort Toh, Tai Chong.
title Osteoinductive effects of 1α, 25 Dihydroxyvitamin D3 on human mesenchymal stem cells.
title_short Osteoinductive effects of 1α, 25 Dihydroxyvitamin D3 on human mesenchymal stem cells.
title_full Osteoinductive effects of 1α, 25 Dihydroxyvitamin D3 on human mesenchymal stem cells.
title_fullStr Osteoinductive effects of 1α, 25 Dihydroxyvitamin D3 on human mesenchymal stem cells.
title_full_unstemmed Osteoinductive effects of 1α, 25 Dihydroxyvitamin D3 on human mesenchymal stem cells.
title_sort osteoinductive effects of 1α, 25 dihydroxyvitamin d3 on human mesenchymal stem cells.
publishDate 2009
url http://hdl.handle.net/10356/15457
_version_ 1759855663080538112