Robust fabrication of composite 3D scaffolds with tissue-specific bioactivity : a proof-of-concept study
The basic requirement of any engineered scaffold is to mimic the native tissue extracellular matrix (ECM). Despite substantial strides in understanding the ECM, scaffold fabrication processes of sufficient product robustness and bioactivity require further investigation, owing to the complexity of t...
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sg-ntu-dr.10356-1546422021-12-30T04:17:09Z Robust fabrication of composite 3D scaffolds with tissue-specific bioactivity : a proof-of-concept study Krishnamoorthi, Muthu Kumar Sarig, Udi Baruch, Limor Ting, Sherwin Reuveny, Shaul Oh, Steve Goldfracht, Idit Gepstein, Lior Venkatraman, Subbu Subramanian Tan, Lay Poh Machluf, Marcelle School of Materials Science and Engineering Engineering::Materials Cardiac ECM Wet Electrospinning The basic requirement of any engineered scaffold is to mimic the native tissue extracellular matrix (ECM). Despite substantial strides in understanding the ECM, scaffold fabrication processes of sufficient product robustness and bioactivity require further investigation, owing to the complexity of the natural ECM. A promising bioacive platform for cardiac tissue engineering is that of decellularized porcine cardiac ECM (pcECM, used here as a soft tissue representative model). However, this platform's complexity and batch-to-batch variability serve as processing limitations in attaining a robust and tunable cardiac tissue-specific bioactive scaffold. To address these issues, we fabricated 3D composite scaffolds (3DCSs) that demonstrate comparable physical and biochemical properties to the natural pcECM using wet electrospinning and functionalization with a pcECM hydrogel. The fabricated 3DCSs are non-immunogenic in vitro and support human mesenchymal stem cells' proliferation. Most importantly, the 3DCSs demonstrate tissue-specific bioactivity in inducing spontaneous cardiac lineage differentiation in human induced pluripotent stem cells (hiPSC) and further support the viability, functionality, and maturation of hiPSC-derived cardiomyocytes. Overall, this work illustrates the technology to fabricate robust yet tunable 3D scaffolds of tissue-specific bioactivity (with a proof of concept provided for cardiac tissues) as a platform for basic materials science studies and possible future R and D application in regenerative medicine. Nanyang Technological University National Research Foundation (NRF) This research is supported by the Singapore National Research Foundation under the CREATE program: The Regenerative Medicine Initiative in Cardiac Restoration Therapy Research, the Li Ka Shing Foundation research grant (grant number 2020LKSFG02A), the Israeli Science Foundation and School of Materials Science & Engineering, Nanyang Technological University, Singapore. 2021-12-30T04:17:09Z 2021-12-30T04:17:09Z 2020 Journal Article Krishnamoorthi, M. K., Sarig, U., Baruch, L., Ting, S., Reuveny, S., Oh, S., Goldfracht, I., Gepstein, L., Venkatraman, S. S., Tan, L. P. & Machluf, M. (2020). Robust fabrication of composite 3D scaffolds with tissue-specific bioactivity : a proof-of-concept study. ACS Applied Bio Materials, 3(8), 4974-4986. https://dx.doi.org/10.1021/acsabm.0c00310 2576-6422 https://hdl.handle.net/10356/154642 10.1021/acsabm.0c00310 2-s2.0-85091000555 8 3 4974 4986 en ACS Applied Bio Materials © 2020 American Chemical Society. All rights reserved. |
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Engineering::Materials Cardiac ECM Wet Electrospinning Krishnamoorthi, Muthu Kumar Sarig, Udi Baruch, Limor Ting, Sherwin Reuveny, Shaul Oh, Steve Goldfracht, Idit Gepstein, Lior Venkatraman, Subbu Subramanian Tan, Lay Poh Machluf, Marcelle Robust fabrication of composite 3D scaffolds with tissue-specific bioactivity : a proof-of-concept study |
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The basic requirement of any engineered scaffold is to mimic the native tissue extracellular matrix (ECM). Despite substantial strides in understanding the ECM, scaffold fabrication processes of sufficient product robustness and bioactivity require further investigation, owing to the complexity of the natural ECM. A promising bioacive platform for cardiac tissue engineering is that of decellularized porcine cardiac ECM (pcECM, used here as a soft tissue representative model). However, this platform's complexity and batch-to-batch variability serve as processing limitations in attaining a robust and tunable cardiac tissue-specific bioactive scaffold. To address these issues, we fabricated 3D composite scaffolds (3DCSs) that demonstrate comparable physical and biochemical properties to the natural pcECM using wet electrospinning and functionalization with a pcECM hydrogel. The fabricated 3DCSs are non-immunogenic in vitro and support human mesenchymal stem cells' proliferation. Most importantly, the 3DCSs demonstrate tissue-specific bioactivity in inducing spontaneous cardiac lineage differentiation in human induced pluripotent stem cells (hiPSC) and further support the viability, functionality, and maturation of hiPSC-derived cardiomyocytes. Overall, this work illustrates the technology to fabricate robust yet tunable 3D scaffolds of tissue-specific bioactivity (with a proof of concept provided for cardiac tissues) as a platform for basic materials science studies and possible future R and D application in regenerative medicine. |
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School of Materials Science and Engineering |
author_facet |
School of Materials Science and Engineering Krishnamoorthi, Muthu Kumar Sarig, Udi Baruch, Limor Ting, Sherwin Reuveny, Shaul Oh, Steve Goldfracht, Idit Gepstein, Lior Venkatraman, Subbu Subramanian Tan, Lay Poh Machluf, Marcelle |
format |
Article |
author |
Krishnamoorthi, Muthu Kumar Sarig, Udi Baruch, Limor Ting, Sherwin Reuveny, Shaul Oh, Steve Goldfracht, Idit Gepstein, Lior Venkatraman, Subbu Subramanian Tan, Lay Poh Machluf, Marcelle |
author_sort |
Krishnamoorthi, Muthu Kumar |
title |
Robust fabrication of composite 3D scaffolds with tissue-specific bioactivity : a proof-of-concept study |
title_short |
Robust fabrication of composite 3D scaffolds with tissue-specific bioactivity : a proof-of-concept study |
title_full |
Robust fabrication of composite 3D scaffolds with tissue-specific bioactivity : a proof-of-concept study |
title_fullStr |
Robust fabrication of composite 3D scaffolds with tissue-specific bioactivity : a proof-of-concept study |
title_full_unstemmed |
Robust fabrication of composite 3D scaffolds with tissue-specific bioactivity : a proof-of-concept study |
title_sort |
robust fabrication of composite 3d scaffolds with tissue-specific bioactivity : a proof-of-concept study |
publishDate |
2021 |
url |
https://hdl.handle.net/10356/154642 |
_version_ |
1722355351128375296 |