Nanomaterials for biomolecules sensing : germanene modified screen printed electrodes for the chronoamperometric detection of histamine in fish samples
Histamine is a biogenic amine that can be found at high concentrations in rotten food such as fish and meat. High histamine consumption could lead to scombroid poisoning that has serious side effects such as hypertension, vomiting and diarrhoea. Hence, this study investigates the chronoamperometr...
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| 格式: | Final Year Project |
| 語言: | English |
| 出版: |
Nanyang Technological University
2022
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| 在線閱讀: | https://hdl.handle.net/10356/154858 |
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| 機構: | Nanyang Technological University |
| 語言: | English |
| 總結: | Histamine is a biogenic amine that can be found at high concentrations in rotten food such as
fish and meat. High histamine consumption could lead to scombroid poisoning that has serious
side effects such as hypertension, vomiting and diarrhoea. Hence, this study investigates the
chronoamperometric detection of histamine using germanene modified screen printed
electrodes (SPE) to allow for sensitive and portable analysis of fish samples during
transportation and storage. To formulate an effective sensor, the electrochemical properties and
stability of histamine were investigated to determine an optimum protocol for
chronoamperometric detection. The characteristics of three germanene nanomaterials were also
investigated to determine the most suitable material for the detection of histamine. Lastly, the
decarboxylation interaction between histamine and diamine oxidase to form imidazole
acetaldehyde was utilised as the main chemical interaction. A secondary interaction also
occurs, whereby the mediator species potassium hexacyanoferrate (III) gets reduced through
its interaction with the newly formed imidazole acetaldehyde. The reduction of potassium
hexacyanoferrate (III) which occurs at the low potentials of +0.2 V was used to indirectly
measure the histamine concentration present in the sample. Through the studies, a
hydrogenated germanene modified SPE with a calibration range of 0.01-150 mgL-1 was
developed and tested on real tuna samples. The sensor proved to be highly effective in
measuring the concentration of histamine present in real tuna samples while also being fast,
cost-efficient, and highly sensitive. |
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