Colorimetric urinalysis for on-site detection of metabolic biomarkers

Over the past few decades, colorimetric assays have been developed for cost-effective and rapid on-site urinalysis. Most of these assays were employed for detection of biomarkers such as glucose, uric acid, ions, and albumin that are abundant in urine at micromolar to millimolar levels. In contrast,...

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Main Authors: Yeasmin, Sanjida, Ammanath, Gopal, Ali, Yusuf, Boehm, Bernhard Otto, Yildiz, Umit Hakan, Palaniappan, Alagappan, Liedberg, Bo
Other Authors: School of Materials Science and Engineering
Format: Article
Language:English
Published: 2022
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Online Access:https://hdl.handle.net/10356/154982
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-1549822022-01-21T04:52:43Z Colorimetric urinalysis for on-site detection of metabolic biomarkers Yeasmin, Sanjida Ammanath, Gopal Ali, Yusuf Boehm, Bernhard Otto Yildiz, Umit Hakan Palaniappan, Alagappan Liedberg, Bo School of Materials Science and Engineering Engineering::Materials Urinalysis Colorimetric Assay Over the past few decades, colorimetric assays have been developed for cost-effective and rapid on-site urinalysis. Most of these assays were employed for detection of biomarkers such as glucose, uric acid, ions, and albumin that are abundant in urine at micromolar to millimolar levels. In contrast, direct assaying of urinary biomarkers such as glycated proteins, low-molecular-weight reactive oxygen species, and nucleic acids that are present at significantly lower levels (nanomolar to picomolar) remain challenging due to the interferences from the urine sample matrix. State-of-the-art assays for detection of trace amounts of urinary biomarkers typically utilize time-consuming and equipment-dependent sample pretreatment or clean-up protocols prior to assaying, which limits their applicability for on-site analysis. Herein, we report a colorimetric assay for on-site detection of trace amount of generic biomarkers in urine without involving tedious sample pretreatment protocols. The detection strategy is based on monitoring the changes in optical properties of poly(3-(4-methyl-3'-thienyloxy)propyltriethylammonium bromide) upon interacting with an aptamer or a peptide nucleic acid in the presence and absence of target biomarkers of relevance for the diagnosis of metabolic complications and diabetes. As a proof of concept, this study demonstrates facile assaying of advanced glycation end products, 8-hydroxy-2'-deoxyguanosine and hepatitis B virus DNA in urine samples at clinically relevant concentrations, with limits of detection of ∼850 pM, ∼650 pM, and ∼ 1 nM, respectively. These analytes represent three distinct classes of biomarkers: (i) glycated proteins, (ii) low-molecular-weight reactive oxygen species, and (iii) nucleic acids. Hence, the proposed methodology is applicable for rapid detection of generic biomarkers in urine, without involving sophisticated equipment and skilled personnel, thereby enabling on-site urinalysis. At the end of the contribution, we discuss the opportunity to translate the homogeneous assay into a paper-based format. Ministry of Education (MOE) Nanyang Technological University The authors wish to acknowledge funding support from Tier 1, MOE -RG 82/12, and NITHM Exploratory Research grant M4081989.070. B.O.B. is supported by an Ong Tiong Tat Chair Professorship and MOE Tier 1 project 2017-T1-001- 139. 2022-01-21T04:52:42Z 2022-01-21T04:52:42Z 2020 Journal Article Yeasmin, S., Ammanath, G., Ali, Y., Boehm, B. O., Yildiz, U. H., Palaniappan, A. & Liedberg, B. (2020). Colorimetric urinalysis for on-site detection of metabolic biomarkers. ACS Applied Materials and Interfaces, 12, 31270-31281. https://dx.doi.org/10.1021/acsami.0c09179 1944-8244 https://hdl.handle.net/10356/154982 10.1021/acsami.0c09179 32551533 2-s2.0-85088249438 12 31270 31281 en RG 82/12 M4081989.070 2017-T1-001- 139. ACS Applied Materials and Interfaces © 2020 American Chemical Society. All rights reserved.
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Materials
Urinalysis
Colorimetric Assay
spellingShingle Engineering::Materials
Urinalysis
Colorimetric Assay
Yeasmin, Sanjida
Ammanath, Gopal
Ali, Yusuf
Boehm, Bernhard Otto
Yildiz, Umit Hakan
Palaniappan, Alagappan
Liedberg, Bo
Colorimetric urinalysis for on-site detection of metabolic biomarkers
description Over the past few decades, colorimetric assays have been developed for cost-effective and rapid on-site urinalysis. Most of these assays were employed for detection of biomarkers such as glucose, uric acid, ions, and albumin that are abundant in urine at micromolar to millimolar levels. In contrast, direct assaying of urinary biomarkers such as glycated proteins, low-molecular-weight reactive oxygen species, and nucleic acids that are present at significantly lower levels (nanomolar to picomolar) remain challenging due to the interferences from the urine sample matrix. State-of-the-art assays for detection of trace amounts of urinary biomarkers typically utilize time-consuming and equipment-dependent sample pretreatment or clean-up protocols prior to assaying, which limits their applicability for on-site analysis. Herein, we report a colorimetric assay for on-site detection of trace amount of generic biomarkers in urine without involving tedious sample pretreatment protocols. The detection strategy is based on monitoring the changes in optical properties of poly(3-(4-methyl-3'-thienyloxy)propyltriethylammonium bromide) upon interacting with an aptamer or a peptide nucleic acid in the presence and absence of target biomarkers of relevance for the diagnosis of metabolic complications and diabetes. As a proof of concept, this study demonstrates facile assaying of advanced glycation end products, 8-hydroxy-2'-deoxyguanosine and hepatitis B virus DNA in urine samples at clinically relevant concentrations, with limits of detection of ∼850 pM, ∼650 pM, and ∼ 1 nM, respectively. These analytes represent three distinct classes of biomarkers: (i) glycated proteins, (ii) low-molecular-weight reactive oxygen species, and (iii) nucleic acids. Hence, the proposed methodology is applicable for rapid detection of generic biomarkers in urine, without involving sophisticated equipment and skilled personnel, thereby enabling on-site urinalysis. At the end of the contribution, we discuss the opportunity to translate the homogeneous assay into a paper-based format.
author2 School of Materials Science and Engineering
author_facet School of Materials Science and Engineering
Yeasmin, Sanjida
Ammanath, Gopal
Ali, Yusuf
Boehm, Bernhard Otto
Yildiz, Umit Hakan
Palaniappan, Alagappan
Liedberg, Bo
format Article
author Yeasmin, Sanjida
Ammanath, Gopal
Ali, Yusuf
Boehm, Bernhard Otto
Yildiz, Umit Hakan
Palaniappan, Alagappan
Liedberg, Bo
author_sort Yeasmin, Sanjida
title Colorimetric urinalysis for on-site detection of metabolic biomarkers
title_short Colorimetric urinalysis for on-site detection of metabolic biomarkers
title_full Colorimetric urinalysis for on-site detection of metabolic biomarkers
title_fullStr Colorimetric urinalysis for on-site detection of metabolic biomarkers
title_full_unstemmed Colorimetric urinalysis for on-site detection of metabolic biomarkers
title_sort colorimetric urinalysis for on-site detection of metabolic biomarkers
publishDate 2022
url https://hdl.handle.net/10356/154982
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