Self-assembly of solubilized human hair keratins

Human hair keratins have proven to be a viable biomaterial for diverse regenerative applications. However, the most significant characteristic of this material, the ability to self-assemble into nanoscale intermediate filaments, has not been exploited. Herein, we successfully demonstrated the induct...

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Main Authors: Lai, Hui Ying, Setyawati, Magdiel Inggrid, Abdul Rahim Ferhan, Divakarla, Shiva Kamini, Chua, Huei Min, Cho, Nam-Joon, Chrzanowski, Wojciech, Ng, Kee Woei
Other Authors: School of Materials Science and Engineering
Format: Article
Language:English
Published: 2022
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Online Access:https://hdl.handle.net/10356/155074
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1550742022-02-05T20:11:27Z Self-assembly of solubilized human hair keratins Lai, Hui Ying Setyawati, Magdiel Inggrid Abdul Rahim Ferhan Divakarla, Shiva Kamini Chua, Huei Min Cho, Nam-Joon Chrzanowski, Wojciech Ng, Kee Woei School of Materials Science and Engineering Skin Research Institute of Singapore, A*STAR Nanyang Environment and Water Research Institute Environmental Chemistry and Materials Centre Engineering::Materials::Biomaterials Self-Assembly Keratin Coating Biomaterial Nanofiber Human hair keratins have proven to be a viable biomaterial for diverse regenerative applications. However, the most significant characteristic of this material, the ability to self-assemble into nanoscale intermediate filaments, has not been exploited. Herein, we successfully demonstrated the induction of hair-extracted keratin self-assembly in vitro to form dense, homogeneous, and continuous nanofibrous networks. These networks remain hydrolytically stable in vitro for up to 5 days in complete cell culture media and are compatible with primary human dermal fibroblasts and keratinocytes. These results enhance the versatility of human hair keratins for applications where structured assembly is of benefit. Agency for Science, Technology and Research (A*STAR) Accepted version This research is supported by the Agency for Science, Technology and Research (A*STAR) under its Acne and Sebaceous Gland Program & Wound Care Innovation for the Tropics IAF-PP (H17/01/a0/008 & H17/01/a0/0L9). 2022-02-04T08:05:28Z 2022-02-04T08:05:28Z 2021 Journal Article Lai, H. Y., Setyawati, M. I., Abdul Rahim Ferhan, Divakarla, S. K., Chua, H. M., Cho, N., Chrzanowski, W. & Ng, K. W. (2021). Self-assembly of solubilized human hair keratins. ACS Biomaterials Science & Engineering, 7(1), 83-89. https://dx.doi.org/10.1021/acsbiomaterials.0c01507 2373-9878 https://hdl.handle.net/10356/155074 10.1021/acsbiomaterials.0c01507 33356132 2-s2.0-85099038689 1 7 83 89 en H17/01/a0/008 H17/01/a0/0L9 ACS Biomaterials Science & Engineering This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Biomaterials Science & Engineering, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acsbiomaterials.0c01507. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Materials::Biomaterials
Self-Assembly
Keratin
Coating
Biomaterial
Nanofiber
spellingShingle Engineering::Materials::Biomaterials
Self-Assembly
Keratin
Coating
Biomaterial
Nanofiber
Lai, Hui Ying
Setyawati, Magdiel Inggrid
Abdul Rahim Ferhan
Divakarla, Shiva Kamini
Chua, Huei Min
Cho, Nam-Joon
Chrzanowski, Wojciech
Ng, Kee Woei
Self-assembly of solubilized human hair keratins
description Human hair keratins have proven to be a viable biomaterial for diverse regenerative applications. However, the most significant characteristic of this material, the ability to self-assemble into nanoscale intermediate filaments, has not been exploited. Herein, we successfully demonstrated the induction of hair-extracted keratin self-assembly in vitro to form dense, homogeneous, and continuous nanofibrous networks. These networks remain hydrolytically stable in vitro for up to 5 days in complete cell culture media and are compatible with primary human dermal fibroblasts and keratinocytes. These results enhance the versatility of human hair keratins for applications where structured assembly is of benefit.
author2 School of Materials Science and Engineering
author_facet School of Materials Science and Engineering
Lai, Hui Ying
Setyawati, Magdiel Inggrid
Abdul Rahim Ferhan
Divakarla, Shiva Kamini
Chua, Huei Min
Cho, Nam-Joon
Chrzanowski, Wojciech
Ng, Kee Woei
format Article
author Lai, Hui Ying
Setyawati, Magdiel Inggrid
Abdul Rahim Ferhan
Divakarla, Shiva Kamini
Chua, Huei Min
Cho, Nam-Joon
Chrzanowski, Wojciech
Ng, Kee Woei
author_sort Lai, Hui Ying
title Self-assembly of solubilized human hair keratins
title_short Self-assembly of solubilized human hair keratins
title_full Self-assembly of solubilized human hair keratins
title_fullStr Self-assembly of solubilized human hair keratins
title_full_unstemmed Self-assembly of solubilized human hair keratins
title_sort self-assembly of solubilized human hair keratins
publishDate 2022
url https://hdl.handle.net/10356/155074
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