Squid suckerin-spider silk fusion protein hydrogel for stem cell-secretome delivery in chronic wounds
The squid sucker ring teeth, used in tandem with the suckers on the squid’s tentacles to capture prey, is comprised of mainly suckerin, a protein material. Squid suckerin is a promising biopolymer for wound dressings due to its biocompatibility, antibacterial properties, and its ability to form gels...
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2022
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sg-ntu-dr.10356-1552162022-06-06T02:22:21Z Squid suckerin-spider silk fusion protein hydrogel for stem cell-secretome delivery in chronic wounds Koh, Kenrick Wang, Jun Kit Gabryelczyk, Bartosz Tay, Dalton Tan, Nguan Soon Miserez, Ali Interdisciplinary Graduate School (IGS) 2022 MRS Spring Meeting & Exhibit Science::Biological sciences Engineering::Materials Squid Suckerin Protein Hydrogel The squid sucker ring teeth, used in tandem with the suckers on the squid’s tentacles to capture prey, is comprised of mainly suckerin, a protein material. Squid suckerin is a promising biopolymer for wound dressings due to its biocompatibility, antibacterial properties, and its ability to form gels/films of varying mechanical properties. However, issues such as low recombinant solubility and the requirement of harsh photochemical means for gel/film formation hinders suckerin’s suitability for encapsulation and delivery of stem cell-secretome, which is a bioactive yet sensitive therapeutic shown to overcome persistent wound inflammation and accelerate wound healing. Via bioinspired design, we developed a novel squid suckerin-spider silk fusion protein hydrogel, where the fusion of key spider silk sequences conferred high solubility and heat-induced gelation properties that enabled encapsulation of secretome without denaturation. The protein hydrogel is capable of long-term delivery of secretome via protein-protein interaction. Additionally, due to its modular design, we incorporated cell adhesion peptides to promote biocompatibility of the hydrogel. In our results, we demonstrate the secretome-loaded hydrogel potential for clinical use via accelerated healing of excisional chronic wounds in vivo. 2022-06-06T02:18:30Z 2022-06-06T02:18:30Z 2022 Conference Paper Koh, K., Wang, J. K., Gabryelczyk, B., Tay, D., Tan, N. S. & Miserez, A. (2022). Squid suckerin-spider silk fusion protein hydrogel for stem cell-secretome delivery in chronic wounds. 2022 MRS Spring Meeting & Exhibit. https://hdl.handle.net/10356/155216 https://www.mrs.org/meetings-events/spring-meetings-exhibits/2022-mrs-spring-meeting/symposium-sessions en © 2022 Materials Research Society. All rights reserved. |
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Science::Biological sciences Engineering::Materials Squid Suckerin Protein Hydrogel Koh, Kenrick Wang, Jun Kit Gabryelczyk, Bartosz Tay, Dalton Tan, Nguan Soon Miserez, Ali Squid suckerin-spider silk fusion protein hydrogel for stem cell-secretome delivery in chronic wounds |
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The squid sucker ring teeth, used in tandem with the suckers on the squid’s tentacles to capture prey, is comprised of mainly suckerin, a protein material. Squid suckerin is a promising biopolymer for wound dressings due to its biocompatibility, antibacterial properties, and its ability to form gels/films of varying mechanical properties. However, issues such as low recombinant solubility and the requirement of harsh photochemical means for gel/film formation hinders suckerin’s suitability for encapsulation and delivery of stem cell-secretome, which is a bioactive yet sensitive therapeutic shown to overcome persistent wound inflammation and accelerate wound healing. Via bioinspired design, we developed a novel squid suckerin-spider silk fusion protein hydrogel, where the fusion of key spider silk sequences conferred high solubility and heat-induced gelation properties that enabled encapsulation of secretome without denaturation. The protein hydrogel is capable of long-term delivery of secretome via protein-protein interaction. Additionally, due to its modular design, we incorporated cell adhesion peptides to promote biocompatibility of the hydrogel. In our results, we demonstrate the secretome-loaded hydrogel potential for clinical use via accelerated healing of excisional chronic wounds in vivo. |
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Interdisciplinary Graduate School (IGS) |
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Interdisciplinary Graduate School (IGS) Koh, Kenrick Wang, Jun Kit Gabryelczyk, Bartosz Tay, Dalton Tan, Nguan Soon Miserez, Ali |
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Conference or Workshop Item |
author |
Koh, Kenrick Wang, Jun Kit Gabryelczyk, Bartosz Tay, Dalton Tan, Nguan Soon Miserez, Ali |
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Koh, Kenrick |
title |
Squid suckerin-spider silk fusion protein hydrogel for stem cell-secretome delivery in chronic wounds |
title_short |
Squid suckerin-spider silk fusion protein hydrogel for stem cell-secretome delivery in chronic wounds |
title_full |
Squid suckerin-spider silk fusion protein hydrogel for stem cell-secretome delivery in chronic wounds |
title_fullStr |
Squid suckerin-spider silk fusion protein hydrogel for stem cell-secretome delivery in chronic wounds |
title_full_unstemmed |
Squid suckerin-spider silk fusion protein hydrogel for stem cell-secretome delivery in chronic wounds |
title_sort |
squid suckerin-spider silk fusion protein hydrogel for stem cell-secretome delivery in chronic wounds |
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2022 |
url |
https://hdl.handle.net/10356/155216 https://www.mrs.org/meetings-events/spring-meetings-exhibits/2022-mrs-spring-meeting/symposium-sessions |
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