Squid suckerin-spider silk fusion protein hydrogel for stem cell-secretome delivery in chronic wounds

The squid sucker ring teeth, used in tandem with the suckers on the squid’s tentacles to capture prey, is comprised of mainly suckerin, a protein material. Squid suckerin is a promising biopolymer for wound dressings due to its biocompatibility, antibacterial properties, and its ability to form gels...

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Main Authors: Koh, Kenrick, Wang, Jun Kit, Gabryelczyk, Bartosz, Tay, Dalton, Tan, Nguan Soon, Miserez, Ali
Other Authors: Interdisciplinary Graduate School (IGS)
Format: Conference or Workshop Item
Language:English
Published: 2022
Subjects:
Online Access:https://hdl.handle.net/10356/155216
https://www.mrs.org/meetings-events/spring-meetings-exhibits/2022-mrs-spring-meeting/symposium-sessions
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1552162022-06-06T02:22:21Z Squid suckerin-spider silk fusion protein hydrogel for stem cell-secretome delivery in chronic wounds Koh, Kenrick Wang, Jun Kit Gabryelczyk, Bartosz Tay, Dalton Tan, Nguan Soon Miserez, Ali Interdisciplinary Graduate School (IGS) 2022 MRS Spring Meeting & Exhibit Science::Biological sciences Engineering::Materials Squid Suckerin Protein Hydrogel The squid sucker ring teeth, used in tandem with the suckers on the squid’s tentacles to capture prey, is comprised of mainly suckerin, a protein material. Squid suckerin is a promising biopolymer for wound dressings due to its biocompatibility, antibacterial properties, and its ability to form gels/films of varying mechanical properties. However, issues such as low recombinant solubility and the requirement of harsh photochemical means for gel/film formation hinders suckerin’s suitability for encapsulation and delivery of stem cell-secretome, which is a bioactive yet sensitive therapeutic shown to overcome persistent wound inflammation and accelerate wound healing. Via bioinspired design, we developed a novel squid suckerin-spider silk fusion protein hydrogel, where the fusion of key spider silk sequences conferred high solubility and heat-induced gelation properties that enabled encapsulation of secretome without denaturation. The protein hydrogel is capable of long-term delivery of secretome via protein-protein interaction. Additionally, due to its modular design, we incorporated cell adhesion peptides to promote biocompatibility of the hydrogel. In our results, we demonstrate the secretome-loaded hydrogel potential for clinical use via accelerated healing of excisional chronic wounds in vivo. 2022-06-06T02:18:30Z 2022-06-06T02:18:30Z 2022 Conference Paper Koh, K., Wang, J. K., Gabryelczyk, B., Tay, D., Tan, N. S. & Miserez, A. (2022). Squid suckerin-spider silk fusion protein hydrogel for stem cell-secretome delivery in chronic wounds. 2022 MRS Spring Meeting & Exhibit. https://hdl.handle.net/10356/155216 https://www.mrs.org/meetings-events/spring-meetings-exhibits/2022-mrs-spring-meeting/symposium-sessions en © 2022 Materials Research Society. All rights reserved.
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Biological sciences
Engineering::Materials
Squid Suckerin
Protein Hydrogel
spellingShingle Science::Biological sciences
Engineering::Materials
Squid Suckerin
Protein Hydrogel
Koh, Kenrick
Wang, Jun Kit
Gabryelczyk, Bartosz
Tay, Dalton
Tan, Nguan Soon
Miserez, Ali
Squid suckerin-spider silk fusion protein hydrogel for stem cell-secretome delivery in chronic wounds
description The squid sucker ring teeth, used in tandem with the suckers on the squid’s tentacles to capture prey, is comprised of mainly suckerin, a protein material. Squid suckerin is a promising biopolymer for wound dressings due to its biocompatibility, antibacterial properties, and its ability to form gels/films of varying mechanical properties. However, issues such as low recombinant solubility and the requirement of harsh photochemical means for gel/film formation hinders suckerin’s suitability for encapsulation and delivery of stem cell-secretome, which is a bioactive yet sensitive therapeutic shown to overcome persistent wound inflammation and accelerate wound healing. Via bioinspired design, we developed a novel squid suckerin-spider silk fusion protein hydrogel, where the fusion of key spider silk sequences conferred high solubility and heat-induced gelation properties that enabled encapsulation of secretome without denaturation. The protein hydrogel is capable of long-term delivery of secretome via protein-protein interaction. Additionally, due to its modular design, we incorporated cell adhesion peptides to promote biocompatibility of the hydrogel. In our results, we demonstrate the secretome-loaded hydrogel potential for clinical use via accelerated healing of excisional chronic wounds in vivo.
author2 Interdisciplinary Graduate School (IGS)
author_facet Interdisciplinary Graduate School (IGS)
Koh, Kenrick
Wang, Jun Kit
Gabryelczyk, Bartosz
Tay, Dalton
Tan, Nguan Soon
Miserez, Ali
format Conference or Workshop Item
author Koh, Kenrick
Wang, Jun Kit
Gabryelczyk, Bartosz
Tay, Dalton
Tan, Nguan Soon
Miserez, Ali
author_sort Koh, Kenrick
title Squid suckerin-spider silk fusion protein hydrogel for stem cell-secretome delivery in chronic wounds
title_short Squid suckerin-spider silk fusion protein hydrogel for stem cell-secretome delivery in chronic wounds
title_full Squid suckerin-spider silk fusion protein hydrogel for stem cell-secretome delivery in chronic wounds
title_fullStr Squid suckerin-spider silk fusion protein hydrogel for stem cell-secretome delivery in chronic wounds
title_full_unstemmed Squid suckerin-spider silk fusion protein hydrogel for stem cell-secretome delivery in chronic wounds
title_sort squid suckerin-spider silk fusion protein hydrogel for stem cell-secretome delivery in chronic wounds
publishDate 2022
url https://hdl.handle.net/10356/155216
https://www.mrs.org/meetings-events/spring-meetings-exhibits/2022-mrs-spring-meeting/symposium-sessions
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