Mechanistic insights into kindlin function and regulation

Kindlins are FERM domain containing adapter proteins known for their role in integrin inside-out and outside-in activation and signalling. In addition, they mediate various integrin-independent processes. How kindlins perform diverse functions under different cellular contexts is not yet fully eluci...

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Main Author: Levitskaya, Zarina
Other Authors: Tan Suet Mien
Format: Thesis-Doctor of Philosophy
Language:English
Published: Nanyang Technological University 2022
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Online Access:https://hdl.handle.net/10356/155651
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1556512023-02-28T18:31:38Z Mechanistic insights into kindlin function and regulation Levitskaya, Zarina Tan Suet Mien School of Biological Sciences SMTan@ntu.edu.sg Science::Biological sciences::Molecular biology Kindlins are FERM domain containing adapter proteins known for their role in integrin inside-out and outside-in activation and signalling. In addition, they mediate various integrin-independent processes. How kindlins perform diverse functions under different cellular contexts is not yet fully elucidated. Here, regulatory mechanisms behind kindlin and integrin activity with respect to their post-translational modifications and interacting partners are explored. The first part of thesis addresses auto-inhibitory mechanism of kindlin-3 oligomerization. Kindlin-3 trimer formation was validated in vitro, and disruption of trimerization resulted in increased integrin-mediated cell spreading and adhesion. In the second part, newly identified interaction of kindlin-3 is explored, uncovering its role in translational regulation. Overall, findings on intra- and inter-molecular interactions of kindlins have opened up new areas for exploration of their functions and potential implications in disease progression. Doctor of Philosophy 2022-03-10T04:26:20Z 2022-03-10T04:26:20Z 2022 Thesis-Doctor of Philosophy Levitskaya, Z. (2022). Mechanistic insights into kindlin function and regulation. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/155651 https://hdl.handle.net/10356/155651 10.32657/10356/155651 en This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0). application/pdf Nanyang Technological University
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Biological sciences::Molecular biology
spellingShingle Science::Biological sciences::Molecular biology
Levitskaya, Zarina
Mechanistic insights into kindlin function and regulation
description Kindlins are FERM domain containing adapter proteins known for their role in integrin inside-out and outside-in activation and signalling. In addition, they mediate various integrin-independent processes. How kindlins perform diverse functions under different cellular contexts is not yet fully elucidated. Here, regulatory mechanisms behind kindlin and integrin activity with respect to their post-translational modifications and interacting partners are explored. The first part of thesis addresses auto-inhibitory mechanism of kindlin-3 oligomerization. Kindlin-3 trimer formation was validated in vitro, and disruption of trimerization resulted in increased integrin-mediated cell spreading and adhesion. In the second part, newly identified interaction of kindlin-3 is explored, uncovering its role in translational regulation. Overall, findings on intra- and inter-molecular interactions of kindlins have opened up new areas for exploration of their functions and potential implications in disease progression.
author2 Tan Suet Mien
author_facet Tan Suet Mien
Levitskaya, Zarina
format Thesis-Doctor of Philosophy
author Levitskaya, Zarina
author_sort Levitskaya, Zarina
title Mechanistic insights into kindlin function and regulation
title_short Mechanistic insights into kindlin function and regulation
title_full Mechanistic insights into kindlin function and regulation
title_fullStr Mechanistic insights into kindlin function and regulation
title_full_unstemmed Mechanistic insights into kindlin function and regulation
title_sort mechanistic insights into kindlin function and regulation
publisher Nanyang Technological University
publishDate 2022
url https://hdl.handle.net/10356/155651
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