Functional analysis of FoxO1 interacting domain with STAT3

• Main Authors: Low, Xiu Hui, Ma, Wei
• Other Authors: Han, Weiping
• Format: Final Year Project
• Language: English
• Published: 2009
• Subjects: DRNTU::Science::Biological sciences::Molecular biology
• Online Access: http://hdl.handle.net/10356/15663
• Institution: Nanyang Technological University

Obesity is one of the most common disorders in children and adults in the developed countries. The obese and type II diabetic patients in particular are often associated to develop insulin and leptin resistance. However, the mechanism of leptin resistance is not fully known. A recent study suggests that the binding of FoxO1 to STAT3 will inhibit STAT3 from interacting with the SP1/POMC promoter, hence preventing POMC anorexigenic effects in the arcuate nucleus of the hypothalamus. The exact binding sites of FoxO1 binding to STAT3 has yet to be discovered. In this study, deletion constructs were built to map the binding sites of FoxO1 to STAT3. The co-immunoprecipitation of deletion constructs with STAT3 were performed and analyzed the protein-protein interactions through western blot. The results showed that the deletion of the region of Pro124-Lys167 in FoxO1 abolished binding with STAT3 but not for the deletion constructs deleted at regions Pro141-lle170 of ΔFoxO1. This suggests the possible binding sites of FoxO1 to STAT3 lies between Pro124-Gly140.