Cell membrane-coated electrospun fibers enhance keratinocyte growth through cell-type specific interactions

• Main Authors: Chooi, Wai Hon, Dong, Quanbin, Low, Jeremy Zhi Yan, Yuen, Clement, Chin, Jiah Shin, Lin, Junquan, Ong, William, Liu, Quan, Chew, Sing Yian
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Article
• Language: English
• Published: 2022
• Subjects: Engineering::Chemical engineering; Nanofibers; Electrospinning
• Online Access: https://hdl.handle.net/10356/157011
• Institution: Nanyang Technological University

Although cell membrane-coated fiber scaffolds can be useful for regenerative medicine by presenting both cell surface antigens and topographical cues, it remains unknown whether changes in cellular behavior on cell membrane-coated scaffolds are due to specific cell-cell interactions. In this work, the effects of scaffold fiber diameters and surface charges on the cell membrane coating efficiency were explored. Furthermore, fibroblast membrane-coated scaffolds improved the growth of human keratinocytes as compared to red blood cell membrane-coated and plain scaffolds. These results suggest the biofunctionality of cell membrane-coated scaffolds and the specific cell-cell interactions that are preserved to modulate cellular response.