The study on the effects of hydrogen peroxide and antibiotic-antimycotic disinfectants on the decellularization of porcine oesophagus

• Main Author: Yah, Jermaine Rui Ting
• Other Authors: Chian Kerm Sin
• Format: Final Year Project
• Language: English
• Published: Nanyang Technological University 2022
• Subjects: Engineering::Mechanical engineering
• Online Access: https://hdl.handle.net/10356/159178
• Institution: Nanyang Technological University

Decellularized pig scaffolds have shown promise in human organ transplant, which can help solve the global organ transplant shortage. The aim of decellularization is to maintain the extracellular matrix, remove donor cells entirely and retain the mechanical structure. In this project, 20mg/ml of sodium deoxycholate (2% SDC) is used in conjunction with 2mg/ml of sodium dodecyl sulfate (0.2% SDS) as both solutions are known to be effective in the decellularization process. The efficacy of 3% hydrogen peroxide was also tested as an alternative storage method to phosphate-buffered saline + 1% antibiotic-antimycotic. The porcine oesophagi are immersed in 200ml of sodium deoxycholate/ sodium dodecyl sulfate over different durations after which DNA quantification and histological results are compared to test the effectiveness of the decellularization process. The results showed that 3% hydrogen peroxide (H2O2) was effective in preventing microbial contamination and was also able to remove some DNA from the oesophagi while retaining the structure of the ECM. Hence H2O2 can be considered as a possible storage alternative.