Microbiota regulates the turnover kinetics of gut macrophages in health and inflammation

The gut immune system has evolved to co-exist in a mutually beneficial symbiotic relationship with its microflora. Here, using a germ-free fate-mapping mouse model, we provide clear insight into how the enteric commensals determine the kinetics of macrophage turnover. The microbiome density along th...

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Main Authors: Chen, Qi, Nair, Sajith, Ruedl, Christiane
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2022
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Online Access:https://hdl.handle.net/10356/159288
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-1592882023-02-28T17:11:50Z Microbiota regulates the turnover kinetics of gut macrophages in health and inflammation Chen, Qi Nair, Sajith Ruedl, Christiane School of Biological Sciences Science::Biological sciences Biological Marker Cytokine The gut immune system has evolved to co-exist in a mutually beneficial symbiotic relationship with its microflora. Here, using a germ-free fate-mapping mouse model, we provide clear insight into how the enteric commensals determine the kinetics of macrophage turnover. The microbiome density along the gastrointestinal tract defines the persistence of ontogenically diverse macrophages, with the highest numbers of the long-lived F4/80hiTim4+ macrophage subset in the less densely colonized small intestine. Furthermore, the microbiome contributes to a tightly regulated monocyte-dependent replenishment of both long- and short-lived F4/80hi macrophages under homeostatic and inflammatory conditions. In the latter situation, the commensals regulate rapid replenishment of the depleted macrophage niche caused by the intestinal inflammation. The microbial ecosystem imprints a favorable cytokine microenvironment in the intestine to support macrophage survival and monocyte-dependent replenishment. Therefore, the host immune system-commensal cross-talk provides an efficient strategy to assure intestinal homeostasis. Ministry of Education (MOE) Published version This work was supported by a Ministry of Education Tier1 grant awarded to C Ruedl. 2022-06-10T05:50:12Z 2022-06-10T05:50:12Z 2022 Journal Article Chen, Q., Nair, S. & Ruedl, C. (2022). Microbiota regulates the turnover kinetics of gut macrophages in health and inflammation. Life Science Alliance, 5(1), e202101178-. https://dx.doi.org/10.26508/lsa.202101178 2575-1077 https://hdl.handle.net/10356/159288 10.26508/lsa.202101178 34728557 1 5 e202101178 en Life Science Alliance 10.21979/N9/XBXJPP © 2021 Chen et al. This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Biological sciences
Biological Marker
Cytokine
spellingShingle Science::Biological sciences
Biological Marker
Cytokine
Chen, Qi
Nair, Sajith
Ruedl, Christiane
Microbiota regulates the turnover kinetics of gut macrophages in health and inflammation
description The gut immune system has evolved to co-exist in a mutually beneficial symbiotic relationship with its microflora. Here, using a germ-free fate-mapping mouse model, we provide clear insight into how the enteric commensals determine the kinetics of macrophage turnover. The microbiome density along the gastrointestinal tract defines the persistence of ontogenically diverse macrophages, with the highest numbers of the long-lived F4/80hiTim4+ macrophage subset in the less densely colonized small intestine. Furthermore, the microbiome contributes to a tightly regulated monocyte-dependent replenishment of both long- and short-lived F4/80hi macrophages under homeostatic and inflammatory conditions. In the latter situation, the commensals regulate rapid replenishment of the depleted macrophage niche caused by the intestinal inflammation. The microbial ecosystem imprints a favorable cytokine microenvironment in the intestine to support macrophage survival and monocyte-dependent replenishment. Therefore, the host immune system-commensal cross-talk provides an efficient strategy to assure intestinal homeostasis.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Chen, Qi
Nair, Sajith
Ruedl, Christiane
format Article
author Chen, Qi
Nair, Sajith
Ruedl, Christiane
author_sort Chen, Qi
title Microbiota regulates the turnover kinetics of gut macrophages in health and inflammation
title_short Microbiota regulates the turnover kinetics of gut macrophages in health and inflammation
title_full Microbiota regulates the turnover kinetics of gut macrophages in health and inflammation
title_fullStr Microbiota regulates the turnover kinetics of gut macrophages in health and inflammation
title_full_unstemmed Microbiota regulates the turnover kinetics of gut macrophages in health and inflammation
title_sort microbiota regulates the turnover kinetics of gut macrophages in health and inflammation
publishDate 2022
url https://hdl.handle.net/10356/159288
_version_ 1759853737057189888