Ca-mediated alleviation of Cd²⁺ induced toxicity and improved Cd²⁺ biomineralization by Sporosarcina pasteurii

Microbial induced carbonate precipitation has been widely used in the biomineralization of heavy metals Cd2+. However, the low Cd-tolerance of ureolytic bacteria limits the applications with only low Cd2+concentrations. In this study, we discovered a simple approach to significantly enhance the Cd2+...

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Main Authors: Fang, Longyang, Niu, Qijian, Cheng, Liang, Jiang, Jianxiong, Yu, Yang-Yang, Chu, Jian, Achal, Varenyam, You, Tianyan
Other Authors: School of Civil and Environmental Engineering
Format: Article
Language:English
Published: 2022
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Online Access:https://hdl.handle.net/10356/159725
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1597252022-06-29T08:23:45Z Ca-mediated alleviation of Cd²⁺ induced toxicity and improved Cd²⁺ biomineralization by Sporosarcina pasteurii Fang, Longyang Niu, Qijian Cheng, Liang Jiang, Jianxiong Yu, Yang-Yang Chu, Jian Achal, Varenyam You, Tianyan School of Civil and Environmental Engineering Engineering::Environmental engineering Microbial Induced Carbonate Precipitation Biomineralization Microbial induced carbonate precipitation has been widely used in the biomineralization of heavy metals Cd2+. However, the low Cd-tolerance of ureolytic bacteria limits the applications with only low Cd2+concentrations. In this study, we discovered a simple approach to significantly enhance the Cd2+ resistance of ureolytic bacteria through an immediate supplement of Ca2+. The Ca2+ protected the cells by reducing the extracellular and intracellular Cd2+ concentration by about 50%. As a result, the Cd2+ removal efficiency was notably improved by about 100% (52.72% to 99.43%, Cd = 5 mM) with Ca2+ supplement. Moreover, extremely high concentration of Cd2+ could be almost completely removed (99.46% at C0 = 20 mM and 99.60% at C0 = 50 mM) within 24 h. Microstructure analyses indicated that the mineralized precipitates were rhombohedral-shaped CdCO3, CaCO3, and (Ca0.67, Cd0.33)CO3. Furthermore, Ca2+ could also protect ureolytic bacteria against toxicity from other heavy metals. The authors would like to thank the supports from the program of Jiangsu Distinguished Professor and the Innovation/Entrepreneurship Program of Jiangsu Province, Jiangsu Province Key Project of Research and Development Plan (BE2020676), the China Postdoctoral Science Foundation (2020M671359), Initial Research Fund of Highly Specialized Personnel from Jiangsu University (4111370003), Senior Talent Funded Projects of Jiangsu University (17JDG016), and Project of Faculty of Agricultural Equipment of Jiangsu University (NZXB20200209), and Natural Science Foundation of Jiangsu Province (Grant no. BK20200914). 2022-06-29T08:23:45Z 2022-06-29T08:23:45Z 2021 Journal Article Fang, L., Niu, Q., Cheng, L., Jiang, J., Yu, Y., Chu, J., Achal, V. & You, T. (2021). Ca-mediated alleviation of Cd²⁺ induced toxicity and improved Cd²⁺ biomineralization by Sporosarcina pasteurii. Science of the Total Environment, 787, 147627-. https://dx.doi.org/10.1016/j.scitotenv.2021.147627 0048-9697 https://hdl.handle.net/10356/159725 10.1016/j.scitotenv.2021.147627 2-s2.0-85111052653 787 147627 en Science of the Total Environment © 2021 Elsevier B.V. All rights reserved.
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Environmental engineering
Microbial Induced Carbonate Precipitation
Biomineralization
spellingShingle Engineering::Environmental engineering
Microbial Induced Carbonate Precipitation
Biomineralization
Fang, Longyang
Niu, Qijian
Cheng, Liang
Jiang, Jianxiong
Yu, Yang-Yang
Chu, Jian
Achal, Varenyam
You, Tianyan
Ca-mediated alleviation of Cd²⁺ induced toxicity and improved Cd²⁺ biomineralization by Sporosarcina pasteurii
description Microbial induced carbonate precipitation has been widely used in the biomineralization of heavy metals Cd2+. However, the low Cd-tolerance of ureolytic bacteria limits the applications with only low Cd2+concentrations. In this study, we discovered a simple approach to significantly enhance the Cd2+ resistance of ureolytic bacteria through an immediate supplement of Ca2+. The Ca2+ protected the cells by reducing the extracellular and intracellular Cd2+ concentration by about 50%. As a result, the Cd2+ removal efficiency was notably improved by about 100% (52.72% to 99.43%, Cd = 5 mM) with Ca2+ supplement. Moreover, extremely high concentration of Cd2+ could be almost completely removed (99.46% at C0 = 20 mM and 99.60% at C0 = 50 mM) within 24 h. Microstructure analyses indicated that the mineralized precipitates were rhombohedral-shaped CdCO3, CaCO3, and (Ca0.67, Cd0.33)CO3. Furthermore, Ca2+ could also protect ureolytic bacteria against toxicity from other heavy metals.
author2 School of Civil and Environmental Engineering
author_facet School of Civil and Environmental Engineering
Fang, Longyang
Niu, Qijian
Cheng, Liang
Jiang, Jianxiong
Yu, Yang-Yang
Chu, Jian
Achal, Varenyam
You, Tianyan
format Article
author Fang, Longyang
Niu, Qijian
Cheng, Liang
Jiang, Jianxiong
Yu, Yang-Yang
Chu, Jian
Achal, Varenyam
You, Tianyan
author_sort Fang, Longyang
title Ca-mediated alleviation of Cd²⁺ induced toxicity and improved Cd²⁺ biomineralization by Sporosarcina pasteurii
title_short Ca-mediated alleviation of Cd²⁺ induced toxicity and improved Cd²⁺ biomineralization by Sporosarcina pasteurii
title_full Ca-mediated alleviation of Cd²⁺ induced toxicity and improved Cd²⁺ biomineralization by Sporosarcina pasteurii
title_fullStr Ca-mediated alleviation of Cd²⁺ induced toxicity and improved Cd²⁺ biomineralization by Sporosarcina pasteurii
title_full_unstemmed Ca-mediated alleviation of Cd²⁺ induced toxicity and improved Cd²⁺ biomineralization by Sporosarcina pasteurii
title_sort ca-mediated alleviation of cd²⁺ induced toxicity and improved cd²⁺ biomineralization by sporosarcina pasteurii
publishDate 2022
url https://hdl.handle.net/10356/159725
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