Metabolic labeling mediated targeting and thermal killing of Gram-positive bacteria by self-reporting Janus magnetic nanoparticles
Nanoparticles have been widely used in detection and killing of bacteria; however, targeting bacteria is still challenging. Delicate design of nanoparticles is required for simultaneous targeting, detection, and therapeutic functions. Here the use of Au/MnFe2 O4 (Au/MFO) Janus nanoparticles to targe...
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sg-ntu-dr.10356-1602462022-07-18T05:23:10Z Metabolic labeling mediated targeting and thermal killing of Gram-positive bacteria by self-reporting Janus magnetic nanoparticles Hou, Shuai Mahadevegowda, Surendra Hittanahalli Lu, Derong Zhang, Kaixi Chan-Park, Mary B. Duan, Hongwei School of Chemical and Biomedical Engineering Engineering::Materials Bacteria Targeting Janus Nanoparticles Nanoparticles have been widely used in detection and killing of bacteria; however, targeting bacteria is still challenging. Delicate design of nanoparticles is required for simultaneous targeting, detection, and therapeutic functions. Here the use of Au/MnFe2 O4 (Au/MFO) Janus nanoparticles to target Gram-positive bacteria via metabolic labeling is reported and realize integrated self-reporting and thermal killing of bacteria. In these nanoparticles, the Au component is functionalized with tetrazine to target trans-cyclooctene group anchored on bacterial cell wall by metabolic incorporation of d-amino acids, and the MFO part exhibits peroxidase activity, enabling self-reporting of bacteria before treatment. The spatial separation of targeting and reporting functions avoids the deterioration of catalytic activity after surface modification. Also important is that MFO facilitates magnetic separation and magnetic heating, leading to easy enrichment and magnetic thermal therapy of labeled bacteria. This method demonstrates that metabolic labeling with d-amino acids is a promising strategy to specifically target and kill Gram-positive bacteria. Ministry of Education (MOE) This work was funded and supported by Ministry of Education-Singapore (MOE2013-T3-1-002, MOE2018-T3-1-003, MOE2018-T2-2-128, and RG45/18). 2022-07-18T05:23:10Z 2022-07-18T05:23:10Z 2021 Journal Article Hou, S., Mahadevegowda, S. H., Lu, D., Zhang, K., Chan-Park, M. B. & Duan, H. (2021). Metabolic labeling mediated targeting and thermal killing of Gram-positive bacteria by self-reporting Janus magnetic nanoparticles. Small, 17(2), 2006357-. https://dx.doi.org/10.1002/smll.202006357 1613-6810 https://hdl.handle.net/10356/160246 10.1002/smll.202006357 33325629 2-s2.0-85097521032 2 17 2006357 en MOE2013-T3-1-002 MOE2018-T3-1-003 MOE2018-T2-2-128 RG45/18 Small © 2020 Wiley-VCH GmbH. All rights reserved. |
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Engineering::Materials Bacteria Targeting Janus Nanoparticles Hou, Shuai Mahadevegowda, Surendra Hittanahalli Lu, Derong Zhang, Kaixi Chan-Park, Mary B. Duan, Hongwei Metabolic labeling mediated targeting and thermal killing of Gram-positive bacteria by self-reporting Janus magnetic nanoparticles |
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Nanoparticles have been widely used in detection and killing of bacteria; however, targeting bacteria is still challenging. Delicate design of nanoparticles is required for simultaneous targeting, detection, and therapeutic functions. Here the use of Au/MnFe2 O4 (Au/MFO) Janus nanoparticles to target Gram-positive bacteria via metabolic labeling is reported and realize integrated self-reporting and thermal killing of bacteria. In these nanoparticles, the Au component is functionalized with tetrazine to target trans-cyclooctene group anchored on bacterial cell wall by metabolic incorporation of d-amino acids, and the MFO part exhibits peroxidase activity, enabling self-reporting of bacteria before treatment. The spatial separation of targeting and reporting functions avoids the deterioration of catalytic activity after surface modification. Also important is that MFO facilitates magnetic separation and magnetic heating, leading to easy enrichment and magnetic thermal therapy of labeled bacteria. This method demonstrates that metabolic labeling with d-amino acids is a promising strategy to specifically target and kill Gram-positive bacteria. |
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School of Chemical and Biomedical Engineering |
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School of Chemical and Biomedical Engineering Hou, Shuai Mahadevegowda, Surendra Hittanahalli Lu, Derong Zhang, Kaixi Chan-Park, Mary B. Duan, Hongwei |
format |
Article |
author |
Hou, Shuai Mahadevegowda, Surendra Hittanahalli Lu, Derong Zhang, Kaixi Chan-Park, Mary B. Duan, Hongwei |
author_sort |
Hou, Shuai |
title |
Metabolic labeling mediated targeting and thermal killing of Gram-positive bacteria by self-reporting Janus magnetic nanoparticles |
title_short |
Metabolic labeling mediated targeting and thermal killing of Gram-positive bacteria by self-reporting Janus magnetic nanoparticles |
title_full |
Metabolic labeling mediated targeting and thermal killing of Gram-positive bacteria by self-reporting Janus magnetic nanoparticles |
title_fullStr |
Metabolic labeling mediated targeting and thermal killing of Gram-positive bacteria by self-reporting Janus magnetic nanoparticles |
title_full_unstemmed |
Metabolic labeling mediated targeting and thermal killing of Gram-positive bacteria by self-reporting Janus magnetic nanoparticles |
title_sort |
metabolic labeling mediated targeting and thermal killing of gram-positive bacteria by self-reporting janus magnetic nanoparticles |
publishDate |
2022 |
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https://hdl.handle.net/10356/160246 |
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1738844893488349184 |