Inkjet-printed phospholipid bilayers on titanium oxide surfaces: towards functional membrane biointerfaces

Functional biointerfaces hold broad significance for designing cell-responsive medical implants and sensor devices. Solid-supported phospholipid bilayers are a promising class of biological materials to build bioinspired thin-film coatings, as they can facilitate interactions with cell membranes. Ho...

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Main Authors: Meker, Sigalit, Halevi, Oded, Chin, Hokyun, Sut, Tun Naw, Jackman, Joshua A., Tan, Ee-Lin, Potroz, Michael G., Cho, Nam-Joon
Other Authors: School of Materials Science and Engineering
Format: Article
Language:English
Published: 2022
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Online Access:https://hdl.handle.net/10356/160639
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-1606392023-07-14T16:04:41Z Inkjet-printed phospholipid bilayers on titanium oxide surfaces: towards functional membrane biointerfaces Meker, Sigalit Halevi, Oded Chin, Hokyun Sut, Tun Naw Jackman, Joshua A. Tan, Ee-Lin Potroz, Michael G. Cho, Nam-Joon School of Materials Science and Engineering Engineering::Materials Lipid Bilayer Inkjet Printing Functional biointerfaces hold broad significance for designing cell-responsive medical implants and sensor devices. Solid-supported phospholipid bilayers are a promising class of biological materials to build bioinspired thin-film coatings, as they can facilitate interactions with cell membranes. However, it remains challenging to fabricate lipid bilayers on medically relevant materials such as titanium oxide surfaces. There are also limitations in existing bilayer printing capabilities since most approaches are restricted to either deposition alone or to fixed microarray patterning. By combining advances in lipid surface chemistry and on-demand inkjet printing, we demonstrate the direct deposition and patterning of covalently tethered lipid bilayer membranes on titanium oxide surfaces, in ambient conditions and without any surface pretreatment process. The deposition conditions were evaluated by quartz crystal microbalance-dissipation (QCM-D) measurements, with corresponding resonance frequency (Δf) and energy dissipation (ΔD) shifts of around -25 Hz and <1 × 10-6, respectively, that indicated successful bilayer printing. The resulting printed phospholipid bilayers are stable in air and do not collapse following dehydration; through rehydration, the bilayers regain their functional properties, such as lateral mobility (>1 µm2/s diffusion coefficient), according to fluorescence recovery after photobleaching (FRAP) measurements. By taking advantage of the lipid bilayer patterned architectures and the unique features of titanium oxide's photoactivity, we further show how patterned cell culture arrays can be fabricated. Looking forward, this work presents new capabilities to achieve stable lipid bilayer patterns that can potentially be translated into implantable biomedical devices. Ministry of Education (MOE) Published version This work was supported by the Ministry of Education (MOE) in Singapore under Grant AcRF TIER1-2020-T1-002-032 (RG111/20) and by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (No. 2020R1C1C1004385). In addition, this work was supported by the International Research and Development Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (2020K1A3A1A39112724). 2022-07-29T02:07:20Z 2022-07-29T02:07:20Z 2022 Journal Article Meker, S., Halevi, O., Chin, H., Sut, T. N., Jackman, J. A., Tan, E., Potroz, M. G. & Cho, N. (2022). Inkjet-printed phospholipid bilayers on titanium oxide surfaces: towards functional membrane biointerfaces. Membranes, 12(4), 361-. https://dx.doi.org/10.3390/membranes12040361 2077-0375 https://hdl.handle.net/10356/160639 10.3390/membranes12040361 35448333 2-s2.0-85128049271 4 12 361 en 2020-T1-002-032 (RG111/20) Membranes © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Materials
Lipid Bilayer
Inkjet Printing
spellingShingle Engineering::Materials
Lipid Bilayer
Inkjet Printing
Meker, Sigalit
Halevi, Oded
Chin, Hokyun
Sut, Tun Naw
Jackman, Joshua A.
Tan, Ee-Lin
Potroz, Michael G.
Cho, Nam-Joon
Inkjet-printed phospholipid bilayers on titanium oxide surfaces: towards functional membrane biointerfaces
description Functional biointerfaces hold broad significance for designing cell-responsive medical implants and sensor devices. Solid-supported phospholipid bilayers are a promising class of biological materials to build bioinspired thin-film coatings, as they can facilitate interactions with cell membranes. However, it remains challenging to fabricate lipid bilayers on medically relevant materials such as titanium oxide surfaces. There are also limitations in existing bilayer printing capabilities since most approaches are restricted to either deposition alone or to fixed microarray patterning. By combining advances in lipid surface chemistry and on-demand inkjet printing, we demonstrate the direct deposition and patterning of covalently tethered lipid bilayer membranes on titanium oxide surfaces, in ambient conditions and without any surface pretreatment process. The deposition conditions were evaluated by quartz crystal microbalance-dissipation (QCM-D) measurements, with corresponding resonance frequency (Δf) and energy dissipation (ΔD) shifts of around -25 Hz and <1 × 10-6, respectively, that indicated successful bilayer printing. The resulting printed phospholipid bilayers are stable in air and do not collapse following dehydration; through rehydration, the bilayers regain their functional properties, such as lateral mobility (>1 µm2/s diffusion coefficient), according to fluorescence recovery after photobleaching (FRAP) measurements. By taking advantage of the lipid bilayer patterned architectures and the unique features of titanium oxide's photoactivity, we further show how patterned cell culture arrays can be fabricated. Looking forward, this work presents new capabilities to achieve stable lipid bilayer patterns that can potentially be translated into implantable biomedical devices.
author2 School of Materials Science and Engineering
author_facet School of Materials Science and Engineering
Meker, Sigalit
Halevi, Oded
Chin, Hokyun
Sut, Tun Naw
Jackman, Joshua A.
Tan, Ee-Lin
Potroz, Michael G.
Cho, Nam-Joon
format Article
author Meker, Sigalit
Halevi, Oded
Chin, Hokyun
Sut, Tun Naw
Jackman, Joshua A.
Tan, Ee-Lin
Potroz, Michael G.
Cho, Nam-Joon
author_sort Meker, Sigalit
title Inkjet-printed phospholipid bilayers on titanium oxide surfaces: towards functional membrane biointerfaces
title_short Inkjet-printed phospholipid bilayers on titanium oxide surfaces: towards functional membrane biointerfaces
title_full Inkjet-printed phospholipid bilayers on titanium oxide surfaces: towards functional membrane biointerfaces
title_fullStr Inkjet-printed phospholipid bilayers on titanium oxide surfaces: towards functional membrane biointerfaces
title_full_unstemmed Inkjet-printed phospholipid bilayers on titanium oxide surfaces: towards functional membrane biointerfaces
title_sort inkjet-printed phospholipid bilayers on titanium oxide surfaces: towards functional membrane biointerfaces
publishDate 2022
url https://hdl.handle.net/10356/160639
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