Segmenting Growth of Endothelial Cells in 6-Well Plates on an Orbital Shaker for Mechanobiological Studies

• Main Authors: Pang, Kuin T., Ghim, Mean, Arshad, Mehwish, Wang, Xiaomeng, Weinberg, Peter D.
• Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
• Format: Article
• Language: English
• Published: 2022
• Subjects: Science::Medicine; Atherosclerosis; Cell Culture
• Online Access: https://hdl.handle.net/10356/160745
• Institution: Nanyang Technological University

Shear stress imposed on the arterial wall by the flow of blood affects endothelial cell morphology and function. Low magnitude, oscillatory and multidirectional shear stresses have all been postulated to stimulate a pro-atherosclerotic phenotype in endothelial cells, whereas high magnitude and unidi...