Biocementation of soil using non-sterile enriched urease-producing bacteria from activated sludge
Biocement based on microbially induced carbonate precipitation has become a new construction material for soil cementation. One of the key elements of biocement is urease-producing bacteria (UPB). This paper presents a new method to enrich UPB from waste activated sludge (WAS) under a non-sterile co...
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sg-ntu-dr.10356-1611702022-08-17T05:39:43Z Biocementation of soil using non-sterile enriched urease-producing bacteria from activated sludge Yang, Yang Chu, Jian Cao, Bin Liu, Hanlong Cheng, Liang School of Civil and Environmental Engineering Engineering::Civil engineering Non-Sterile Enrichment Biocement based on microbially induced carbonate precipitation has become a new construction material for soil cementation. One of the key elements of biocement is urease-producing bacteria (UPB). This paper presents a new method to enrich UPB from waste activated sludge (WAS) under a non-sterile condition so the cultivation of bacteria can be carried out in large-scale for field applications. Using this method, the cost of bacteria cultivation can be reduced by up to 30%. The UPB culture enriched using this method is highly urease active and capable of hydrolyzing urea for calcium carbonate precipitation. After treating sand columns using biocement made of this type of UPB via equal molar cementation solution of 2 M, the unconfined compressive strength of the sand increased to 2.7 MPa and the permeability reduced to a level of 10−6 m/s. These results are comparable with those obtained for biotreatment using pure UPB. Thus, the UPB cultivating method introduced in this paper has the potential to reduce the cost of soil improvement without compromising the performance. Ministry of Education (MOE) This work was supported by Grant No MOE2015-T2-2-142 provided by the Ministry of Education, Singapore. 2022-08-17T05:39:43Z 2022-08-17T05:39:43Z 2020 Journal Article Yang, Y., Chu, J., Cao, B., Liu, H. & Cheng, L. (2020). Biocementation of soil using non-sterile enriched urease-producing bacteria from activated sludge. Journal of Cleaner Production, 262, 121315-. https://dx.doi.org/10.1016/j.jclepro.2020.121315 0959-6526 https://hdl.handle.net/10356/161170 10.1016/j.jclepro.2020.121315 2-s2.0-85082741960 262 121315 en MOE2015-T2-2-142 Journal of Cleaner Production © 2020 Elsevier Ltd. All rights reserved. |
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Engineering::Civil engineering Non-Sterile Enrichment Yang, Yang Chu, Jian Cao, Bin Liu, Hanlong Cheng, Liang Biocementation of soil using non-sterile enriched urease-producing bacteria from activated sludge |
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Biocement based on microbially induced carbonate precipitation has become a new construction material for soil cementation. One of the key elements of biocement is urease-producing bacteria (UPB). This paper presents a new method to enrich UPB from waste activated sludge (WAS) under a non-sterile condition so the cultivation of bacteria can be carried out in large-scale for field applications. Using this method, the cost of bacteria cultivation can be reduced by up to 30%. The UPB culture enriched using this method is highly urease active and capable of hydrolyzing urea for calcium carbonate precipitation. After treating sand columns using biocement made of this type of UPB via equal molar cementation solution of 2 M, the unconfined compressive strength of the sand increased to 2.7 MPa and the permeability reduced to a level of 10−6 m/s. These results are comparable with those obtained for biotreatment using pure UPB. Thus, the UPB cultivating method introduced in this paper has the potential to reduce the cost of soil improvement without compromising the performance. |
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School of Civil and Environmental Engineering |
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School of Civil and Environmental Engineering Yang, Yang Chu, Jian Cao, Bin Liu, Hanlong Cheng, Liang |
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Article |
author |
Yang, Yang Chu, Jian Cao, Bin Liu, Hanlong Cheng, Liang |
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Yang, Yang |
title |
Biocementation of soil using non-sterile enriched urease-producing bacteria from activated sludge |
title_short |
Biocementation of soil using non-sterile enriched urease-producing bacteria from activated sludge |
title_full |
Biocementation of soil using non-sterile enriched urease-producing bacteria from activated sludge |
title_fullStr |
Biocementation of soil using non-sterile enriched urease-producing bacteria from activated sludge |
title_full_unstemmed |
Biocementation of soil using non-sterile enriched urease-producing bacteria from activated sludge |
title_sort |
biocementation of soil using non-sterile enriched urease-producing bacteria from activated sludge |
publishDate |
2022 |
url |
https://hdl.handle.net/10356/161170 |
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1743119470587543552 |