Ordered micropillar array gold electrode increases electrochemical signature of early biofilm attachment

Extracellular electron transfer (EET) from microorganisms to insoluble metals and electrodes is relevant to energy recovery from wastewater, green production of high-added value chemicals, and biosensors for food, environmental, and clinical applications. Microstructured electrode surfaces increase...

Full description

Saved in:
Bibliographic Details
Main Authors: Astorga, Solange Elizabeth, Hu, Liangxing, Marsili, Enrico, Huang, Yizhong
Other Authors: School of Materials Science and Engineering
Format: Article
Language:English
Published: 2022
Subjects:
Online Access:https://hdl.handle.net/10356/161447
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Nanyang Technological University
Language: English
id sg-ntu-dr.10356-161447
record_format dspace
spelling sg-ntu-dr.10356-1614472022-09-10T23:31:41Z Ordered micropillar array gold electrode increases electrochemical signature of early biofilm attachment Astorga, Solange Elizabeth Hu, Liangxing Marsili, Enrico Huang, Yizhong School of Materials Science and Engineering School of Mechanical and Aerospace Engineering Singapore Centre for Environmental Life Sciences and Engineering (SCELSE) Engineering::Materials Bioelectrochemistry Extracellular Electron Transfer Extracellular electron transfer (EET) from microorganisms to insoluble metals and electrodes is relevant to energy recovery from wastewater, green production of high-added value chemicals, and biosensors for food, environmental, and clinical applications. Microstructured electrode surfaces increase EET rate in bioelectrochemical systems, thus enabling higher sensibility and power output as well as the detection of bacteria and biofilms in bioelectrochemical sensors. However, many aspects of the EET process, particularly in early biofilm stages, are still poorly understood. We report a microstructured gold electrode maintained at oxidative potential to support the growth of Escherichia coli, measure the electrochemical output, and analyze the EET rate during early biofilm formation. The charge outputs of the modified electrodes are up to 22% higher than the control electrodes, enabling the electrochemical detection of early E. coli biofilms. The electrode microstructures promote biofilm attachment, as confirmed by field emission scanning electron microscope (FESEM) and confocal laser scanning microscope (CLSM) imaging. Following biofilm formation, the resistance to charge transfer at the biofilm-electrode interface decreases and the capacitance increases as shown by EIS analysis. Overall, these results contribute to the understanding of EET in early biofilms, towards developing sensitive bioelectrochemical sensors for biofilm detection. Ministry of Education (MOE) Nanyang Technological University National Research Foundation (NRF) Published version This work was supported by Singapore Centre for Environmental Life Sciences Engineering (SCELSE), whose research is supported by the National Research Foundation Singapore, Ministry of Education, Nanyang Technological University and National University of Singapore, under its Research Centre of Excellence Program. Additional support was provided by Material Science and Engineering, Nanyang Technological University, Tier 1 by the National Research Foundation Singapore, Ministry of Education (MOE) [M4011959 and M4011528]. Solange Elizabeth Astorgawas partially supported by a Roberto Rocca fellowship. 2022-09-02T04:56:03Z 2022-09-02T04:56:03Z 2020 Journal Article Astorga, S. E., Hu, L., Marsili, E. & Huang, Y. (2020). Ordered micropillar array gold electrode increases electrochemical signature of early biofilm attachment. Materials & Design, 185, 108256-. https://dx.doi.org/10.1016/j.matdes.2019.108256 0261-3069 https://hdl.handle.net/10356/161447 10.1016/j.matdes.2019.108256 2-s2.0-85073107711 185 108256 en M4011959 M4011528 Materials & Design © 2019 Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Materials
Bioelectrochemistry
Extracellular Electron Transfer
spellingShingle Engineering::Materials
Bioelectrochemistry
Extracellular Electron Transfer
Astorga, Solange Elizabeth
Hu, Liangxing
Marsili, Enrico
Huang, Yizhong
Ordered micropillar array gold electrode increases electrochemical signature of early biofilm attachment
description Extracellular electron transfer (EET) from microorganisms to insoluble metals and electrodes is relevant to energy recovery from wastewater, green production of high-added value chemicals, and biosensors for food, environmental, and clinical applications. Microstructured electrode surfaces increase EET rate in bioelectrochemical systems, thus enabling higher sensibility and power output as well as the detection of bacteria and biofilms in bioelectrochemical sensors. However, many aspects of the EET process, particularly in early biofilm stages, are still poorly understood. We report a microstructured gold electrode maintained at oxidative potential to support the growth of Escherichia coli, measure the electrochemical output, and analyze the EET rate during early biofilm formation. The charge outputs of the modified electrodes are up to 22% higher than the control electrodes, enabling the electrochemical detection of early E. coli biofilms. The electrode microstructures promote biofilm attachment, as confirmed by field emission scanning electron microscope (FESEM) and confocal laser scanning microscope (CLSM) imaging. Following biofilm formation, the resistance to charge transfer at the biofilm-electrode interface decreases and the capacitance increases as shown by EIS analysis. Overall, these results contribute to the understanding of EET in early biofilms, towards developing sensitive bioelectrochemical sensors for biofilm detection.
author2 School of Materials Science and Engineering
author_facet School of Materials Science and Engineering
Astorga, Solange Elizabeth
Hu, Liangxing
Marsili, Enrico
Huang, Yizhong
format Article
author Astorga, Solange Elizabeth
Hu, Liangxing
Marsili, Enrico
Huang, Yizhong
author_sort Astorga, Solange Elizabeth
title Ordered micropillar array gold electrode increases electrochemical signature of early biofilm attachment
title_short Ordered micropillar array gold electrode increases electrochemical signature of early biofilm attachment
title_full Ordered micropillar array gold electrode increases electrochemical signature of early biofilm attachment
title_fullStr Ordered micropillar array gold electrode increases electrochemical signature of early biofilm attachment
title_full_unstemmed Ordered micropillar array gold electrode increases electrochemical signature of early biofilm attachment
title_sort ordered micropillar array gold electrode increases electrochemical signature of early biofilm attachment
publishDate 2022
url https://hdl.handle.net/10356/161447
_version_ 1744365368142462976