Bioinformatics study of sulphate-reducing bacteria in environmental engineering

• Main Author: Tng, Gina Huishan.
• Other Authors: Maszenan Bin Abdul Majid
• Format: Final Year Project
• Language: English
• Published: 2009
• Subjects: DRNTU::Engineering::Environmental engineering
• Online Access: http://hdl.handle.net/10356/16156
• Institution: Nanyang Technological University

Microorganisms are very tiny unicellular (one-cell) organisms. Examples are viruses, fungi, and bacteria which are found practically everywhere in this world. Their existence occurs in all kinds of environments such as in living things in plants and animals. They occur in huge quantities that they make up more than the cells of a human body which is approximately ten times more. Microorganisms are classified into different groups in accordance to their environmental temperature, the prokaryote or eukaryote group as well as their genotype. In this project, triplet ratio of bacteria is used to differentiate and classify them at family, genus and species level. Analysis of the triplet ratio using two dimensional graphs will be used. Sulphate-reducing bacteria are used as a prime example, to be examined and classified using the analysis of the triplet ratio in the sequencing of 16s RNA. Through this research, different groups of sulpate-reducing bacteria can be differentiated from one another. This will in turn yield new understanding of their fundamental biochemical mechanism which could be used for future development of their environmental roles. This will lead to better understanding of classification of bacteria using the triplet ratio and application of bioinformatics in future classification of microorganisms.