Elucidating membrane proteomic responses of Escherichia coli to alcohol stress
Bioalcohol is a renewable and environment-friendly alternative to present day fossil fuels, which are non-renewable and greenhouse gas emitters. However, alcohol toxicity to microbial cells is the limiting factor to profitable, large-scale production of alcohol by fermentation. An in-depth unders...
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| Format: | Thesis-Doctor of Philosophy |
| Language: | English |
| Published: |
Nanyang Technological University
2022
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| Online Access: | https://hdl.handle.net/10356/161698 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | Bioalcohol is a renewable and environment-friendly alternative to present day
fossil fuels, which are non-renewable and greenhouse gas emitters. However,
alcohol toxicity to microbial cells is the limiting factor to profitable, large-scale
production of alcohol by fermentation. An in-depth understanding of how the
bacterial cells respond to alcohol stress by altering its membrane proteome can
provide valuable information, which might be useful in improving microbial
tolerance to alcohol and hence enable large scale production. The current study
deals with the understanding of changes in the membrane protein composition of
Escherichia coli in the presence of ethanol, butanol and hexanol. Further, the
study focusses on confirming the changes in the expression of important outer
membrane proteins under alcohol stress and predict the effect of such response
on the functional integrity of the outer membrane. |
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