Development of reconstructed intestinal micronucleus cytome (RICyt) assay in 3D human gut model for genotoxicity assessment of orally ingested substances

• Main Authors: Lim, Hui Kheng, Hughes, Christopher Owen, Lim, Michelle Jing Sin, Li, Jia'En Jasmine, Rakshit, Moumita, Yeo, Calvin, Chng, Kern Rei, Li, Angela, Chan, Joanne Sheot Harn, Ng, Kee Woei, Leavesley, David Ian, Smith, Benjamin Paul Chapman
• Other Authors: School of Materials Science and Engineering
• Format: Article
• Language: English
• Published: 2022
• Subjects: Engineering::Materials; Micronuclei; DNA Damage
• Online Access: https://hdl.handle.net/10356/161945
• Institution: Nanyang Technological University

The micronucleus (MN) assay is widely used as part of a battery of tests applied to evaluate the genotoxic potential of chemicals, including new food additives and novel food ingredients. Micronucleus assays typically utilise homogenous in vitro cell lines which poorly recapitulate the physiology, b...