Quantification of intrinsic optical signals in the outer human retina using optical coherence tomography
Intrinsic optical signals constitute a noninvasive biomarker promising the objective assessment of retinal photoreceptor function. We employed a commercial optical coherence tomography (OCT) system and an OCT signal model for evaluation of optical path length (OPL) changes in the temporal outer reti...
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sg-ntu-dr.10356-1626042023-12-29T06:48:57Z Quantification of intrinsic optical signals in the outer human retina using optical coherence tomography Messner, Alina Dos Santos, Valentin Aranha Stegmann, Hannes Puchner, Stefan Schmidl, Doreen Leitgeb, Rainer Schmetterer, Leopold Werkmeister, René M. School of Chemical and Biomedical Engineering Singapore Eye Research Institute, The Academia, Singapore. Duke-NUS Medical School SERI-NTU Advanced Ocular Engineering (STANCE) Program Engineering::Bioengineering Intrinsic Optical Signals Optical Coherence Tomography Intrinsic optical signals constitute a noninvasive biomarker promising the objective assessment of retinal photoreceptor function. We employed a commercial optical coherence tomography (OCT) system and an OCT signal model for evaluation of optical path length (OPL) changes in the temporal outer retina of five healthy subjects during light adaptation. Data were acquired at 30 time points, in ambient light and during long duration stimulation with white light, and analyzed, employing a signal model based on the sum of seven Gaussian curves corresponding to all relevant anatomical structures of the outer retina. During light stimulation, mean OPL between rod outer segment tips (ROST) and the retinal pigment epithelium (RPE) decreased by 21.4 ± 3.5%. Further, OPL between the external-limiting membrane (ELM) and the RPE decreased by 5.2 ± 0.9% versus baseline, while OPL between ELM and ROST showed an initial decrease by 2.1 ± 1.6% versus baseline and, thereafter, increased by 2.8 ± 2.1% versus baseline. Thus, the presented approach allowed for assess to dynamic changes in the outer retina in response to light. The change in the subretinal space occurring in the context of light adaptation could be measured using a standard OCT platform and a dedicated signal model. Published version The research has been funded by the Vienna Science and Technology Fund (WWTF) through project LS14-067. 2022-11-01T02:12:58Z 2022-11-01T02:12:58Z 2022 Journal Article Messner, A., Dos Santos, V. A., Stegmann, H., Puchner, S., Schmidl, D., Leitgeb, R., Schmetterer, L. & Werkmeister, R. M. (2022). Quantification of intrinsic optical signals in the outer human retina using optical coherence tomography. Annals of the New York Academy of Sciences, 1510(1), 145-157. https://dx.doi.org/10.1111/nyas.14721 0077-8923 https://hdl.handle.net/10356/162604 10.1111/nyas.14721 34893981 2-s2.0-85128082816 1 1510 145 157 en Annals of the New York Academy of Sciences © 2021 The Authors. Annals of the New York Academy of Sciences published by Wiley Periodicals LLC on behalf of New York Academy of Sciences. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. application/pdf |
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Engineering::Bioengineering Intrinsic Optical Signals Optical Coherence Tomography Messner, Alina Dos Santos, Valentin Aranha Stegmann, Hannes Puchner, Stefan Schmidl, Doreen Leitgeb, Rainer Schmetterer, Leopold Werkmeister, René M. Quantification of intrinsic optical signals in the outer human retina using optical coherence tomography |
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Intrinsic optical signals constitute a noninvasive biomarker promising the objective assessment of retinal photoreceptor function. We employed a commercial optical coherence tomography (OCT) system and an OCT signal model for evaluation of optical path length (OPL) changes in the temporal outer retina of five healthy subjects during light adaptation. Data were acquired at 30 time points, in ambient light and during long duration stimulation with white light, and analyzed, employing a signal model based on the sum of seven Gaussian curves corresponding to all relevant anatomical structures of the outer retina. During light stimulation, mean OPL between rod outer segment tips (ROST) and the retinal pigment epithelium (RPE) decreased by 21.4 ± 3.5%. Further, OPL between the external-limiting membrane (ELM) and the RPE decreased by 5.2 ± 0.9% versus baseline, while OPL between ELM and ROST showed an initial decrease by 2.1 ± 1.6% versus baseline and, thereafter, increased by 2.8 ± 2.1% versus baseline. Thus, the presented approach allowed for assess to dynamic changes in the outer retina in response to light. The change in the subretinal space occurring in the context of light adaptation could be measured using a standard OCT platform and a dedicated signal model. |
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School of Chemical and Biomedical Engineering |
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School of Chemical and Biomedical Engineering Messner, Alina Dos Santos, Valentin Aranha Stegmann, Hannes Puchner, Stefan Schmidl, Doreen Leitgeb, Rainer Schmetterer, Leopold Werkmeister, René M. |
format |
Article |
author |
Messner, Alina Dos Santos, Valentin Aranha Stegmann, Hannes Puchner, Stefan Schmidl, Doreen Leitgeb, Rainer Schmetterer, Leopold Werkmeister, René M. |
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Messner, Alina |
title |
Quantification of intrinsic optical signals in the outer human retina using optical coherence tomography |
title_short |
Quantification of intrinsic optical signals in the outer human retina using optical coherence tomography |
title_full |
Quantification of intrinsic optical signals in the outer human retina using optical coherence tomography |
title_fullStr |
Quantification of intrinsic optical signals in the outer human retina using optical coherence tomography |
title_full_unstemmed |
Quantification of intrinsic optical signals in the outer human retina using optical coherence tomography |
title_sort |
quantification of intrinsic optical signals in the outer human retina using optical coherence tomography |
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2022 |
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https://hdl.handle.net/10356/162604 |
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