Improving printability of hydrogel-based bio-inks for thermal inkjet bioprinting applications via saponification and heat treatment processes

Material jetting bioprinting is a highly promising three-dimensional (3D) bioprinting technique that facilitates drop-on-demand (DOD) deposition of biomaterials and cells at pre-defined positions with high precision and resolution. A major challenge that hinders the prevalent use of the material jet...

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Main Authors: Suntornnond, Ratima, Ng, Wei Long, Huang, Xi, Yeow, Ethan Chuen Herh, Yeong, Wai Yee
Other Authors: School of Mechanical and Aerospace Engineering
Format: Article
Language:English
Published: 2022
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Online Access:https://hdl.handle.net/10356/162856
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1628562022-11-12T23:31:50Z Improving printability of hydrogel-based bio-inks for thermal inkjet bioprinting applications via saponification and heat treatment processes Suntornnond, Ratima Ng, Wei Long Huang, Xi Yeow, Ethan Chuen Herh Yeong, Wai Yee School of Mechanical and Aerospace Engineering Singapore Centre for 3D Printing HP-NTU Digital Manufacturing Corporate Lab Engineering::Materials Bioprinting Hydrogels Material jetting bioprinting is a highly promising three-dimensional (3D) bioprinting technique that facilitates drop-on-demand (DOD) deposition of biomaterials and cells at pre-defined positions with high precision and resolution. A major challenge that hinders the prevalent use of the material jetting bioprinting technique is due to its limited range of printable hydrogel-based bio-inks. As a proof-of-concept, further modifications were made to gelatin methacrylate (GelMA), a gold-standard bio-ink, to improve its printability in a thermal inkjet bioprinter (HP Inc. D300e Digital Dispenser). A two-step modification process comprising saponification and heat treatment was performed; the GelMA bio-ink was first modified via a saponification process under highly alkali conditions to obtain saponified GelMA (SP-GelMA), followed by heat treatment via an autoclaving process to obtain heat-treated SP-GelMA (HSP-GelMA). The bio-ink modification process was optimized by evaluating the material properties of the GelMA bio-inks via rheological characterization, the bio-ink crosslinking test, nuclear magnetic resonance (NMR) spectroscopy and the material swelling ratio after different numbers of heat treatment cycles (0, 1, 2 and 3 cycles). Lastly, size-exclusion chromatography with multi-angle light scattering (SEC-MALS) was performed to determine the effect of heat treatment on the molecular weight of the bio-inks. In this work, the 4% H2SP-GelMA bio-inks (after 2 heat treatment cycles) demonstrated good printability and biocompatibility (in terms of cell viability and proliferation profile). Furthermore, thermal inkjet bioprinting of the modified hydrogel-based bio-ink (a two-step modification process comprising saponification and heat treatment) via direct/indirect cell patterning is a facile approach for potential fundamental cell-cell and cell-material interaction studies. Submitted/Accepted version This study was supported by the RIE2020 Industry Alignment Fund – Industry Collaboration Projects (IAF-ICP) Funding Initiative, as well as cash and in-kind contribution from the industry partner, HP Inc., through the HP-NTU Digital Manufacturing Corporate Lab. 2022-11-11T02:55:03Z 2022-11-11T02:55:03Z 2022 Journal Article Suntornnond, R., Ng, W. L., Huang, X., Yeow, E. C. H. & Yeong, W. Y. (2022). Improving printability of hydrogel-based bio-inks for thermal inkjet bioprinting applications via saponification and heat treatment processes. Journal of Materials Chemistry B, 10(31), 5989-6000. https://dx.doi.org/10.1039/d2tb00442a 2050-750X https://hdl.handle.net/10356/162856 10.1039/d2tb00442a 35876487 2-s2.0-85135289629 31 10 5989 6000 en IAF-ICP Journal of Materials Chemistry B © 2022 The Royal Society of Chemistry. All rights reserved. This paper was published in Journal of Materials Chemistry B and is made available with permission of The Royal Society of Chemistry. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Materials
Bioprinting
Hydrogels
spellingShingle Engineering::Materials
Bioprinting
Hydrogels
Suntornnond, Ratima
Ng, Wei Long
Huang, Xi
Yeow, Ethan Chuen Herh
Yeong, Wai Yee
Improving printability of hydrogel-based bio-inks for thermal inkjet bioprinting applications via saponification and heat treatment processes
description Material jetting bioprinting is a highly promising three-dimensional (3D) bioprinting technique that facilitates drop-on-demand (DOD) deposition of biomaterials and cells at pre-defined positions with high precision and resolution. A major challenge that hinders the prevalent use of the material jetting bioprinting technique is due to its limited range of printable hydrogel-based bio-inks. As a proof-of-concept, further modifications were made to gelatin methacrylate (GelMA), a gold-standard bio-ink, to improve its printability in a thermal inkjet bioprinter (HP Inc. D300e Digital Dispenser). A two-step modification process comprising saponification and heat treatment was performed; the GelMA bio-ink was first modified via a saponification process under highly alkali conditions to obtain saponified GelMA (SP-GelMA), followed by heat treatment via an autoclaving process to obtain heat-treated SP-GelMA (HSP-GelMA). The bio-ink modification process was optimized by evaluating the material properties of the GelMA bio-inks via rheological characterization, the bio-ink crosslinking test, nuclear magnetic resonance (NMR) spectroscopy and the material swelling ratio after different numbers of heat treatment cycles (0, 1, 2 and 3 cycles). Lastly, size-exclusion chromatography with multi-angle light scattering (SEC-MALS) was performed to determine the effect of heat treatment on the molecular weight of the bio-inks. In this work, the 4% H2SP-GelMA bio-inks (after 2 heat treatment cycles) demonstrated good printability and biocompatibility (in terms of cell viability and proliferation profile). Furthermore, thermal inkjet bioprinting of the modified hydrogel-based bio-ink (a two-step modification process comprising saponification and heat treatment) via direct/indirect cell patterning is a facile approach for potential fundamental cell-cell and cell-material interaction studies.
author2 School of Mechanical and Aerospace Engineering
author_facet School of Mechanical and Aerospace Engineering
Suntornnond, Ratima
Ng, Wei Long
Huang, Xi
Yeow, Ethan Chuen Herh
Yeong, Wai Yee
format Article
author Suntornnond, Ratima
Ng, Wei Long
Huang, Xi
Yeow, Ethan Chuen Herh
Yeong, Wai Yee
author_sort Suntornnond, Ratima
title Improving printability of hydrogel-based bio-inks for thermal inkjet bioprinting applications via saponification and heat treatment processes
title_short Improving printability of hydrogel-based bio-inks for thermal inkjet bioprinting applications via saponification and heat treatment processes
title_full Improving printability of hydrogel-based bio-inks for thermal inkjet bioprinting applications via saponification and heat treatment processes
title_fullStr Improving printability of hydrogel-based bio-inks for thermal inkjet bioprinting applications via saponification and heat treatment processes
title_full_unstemmed Improving printability of hydrogel-based bio-inks for thermal inkjet bioprinting applications via saponification and heat treatment processes
title_sort improving printability of hydrogel-based bio-inks for thermal inkjet bioprinting applications via saponification and heat treatment processes
publishDate 2022
url https://hdl.handle.net/10356/162856
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