Tetraspanin Cd9b and Cxcl12a/Cxcr4b have a synergistic effect on the control of collective cell migration

Collective cell migration is essential for embryonic development and homeostatic processes. During zebrafish development, the posterior lateral line primordium (pLLP) navigates along the embryo flank by collective cell migration. The chemokine receptors, Cxcr4b and Cxcr7b, as well as their cognate l...

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Main Authors: Marsay, Katherine S., Greaves, Sarah, Mahabaleshwar, Harsha, Ho, Charmaine Min, Roehl, Henry, Monk, Peter N., Carney, Tom J., Partridge, Lynda J.
Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
Format: Article
Language:English
Published: 2022
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Online Access:https://hdl.handle.net/10356/163099
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1630992023-03-05T16:51:58Z Tetraspanin Cd9b and Cxcl12a/Cxcr4b have a synergistic effect on the control of collective cell migration Marsay, Katherine S. Greaves, Sarah Mahabaleshwar, Harsha Ho, Charmaine Min Roehl, Henry Monk, Peter N. Carney, Tom J. Partridge, Lynda J. Lee Kong Chian School of Medicine (LKCMedicine) Institute of Molecular and Cell Biology, A*STAR Science::Medicine Protein Cd9b Protein Cxcl12a Collective cell migration is essential for embryonic development and homeostatic processes. During zebrafish development, the posterior lateral line primordium (pLLP) navigates along the embryo flank by collective cell migration. The chemokine receptors, Cxcr4b and Cxcr7b, as well as their cognate ligand, Cxcl12a, are essential for this process. We corroborate that knockdown of the zebrafish cd9 tetraspanin orthologue, cd9b, results in mild pLL abnormalities. Through generation of CRISPR and TALEN mutants, we show that cd9a and cd9b function partially redundantly in pLLP migration, which is delayed in the cd9b single and cd9a; cd9b double mutants. This delay led to a transient reduction in neuromast numbers. Loss of both Cd9a and Cd9b sensitized embryos to reduced Cxcr4b and Cxcl12a levels. Together these results provide evidence that Cd9 modulates collective cell migration of the pLLP during zebrafish development. One interpretation of these observations is that Cd9 contributes to more effective chemokine signalling. Published version The project was partly funded under the grant HR received from the MRC (MR/J001457/1). KM was funded by a studentship from IMCB-A-STAR Singapore and the University of Sheffield. 2022-11-21T06:33:38Z 2022-11-21T06:33:38Z 2021 Journal Article Marsay, K. S., Greaves, S., Mahabaleshwar, H., Ho, C. M., Roehl, H., Monk, P. N., Carney, T. J. & Partridge, L. J. (2021). Tetraspanin Cd9b and Cxcl12a/Cxcr4b have a synergistic effect on the control of collective cell migration. PloS One, 16(11), e0260372-. https://dx.doi.org/10.1371/journal.pone.0260372 1932-6203 https://hdl.handle.net/10356/163099 10.1371/journal.pone.0260372 34847198 2-s2.0-85120427143 11 16 e0260372 en PloS one © 2021 Marsay et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Medicine
Protein Cd9b
Protein Cxcl12a
spellingShingle Science::Medicine
Protein Cd9b
Protein Cxcl12a
Marsay, Katherine S.
Greaves, Sarah
Mahabaleshwar, Harsha
Ho, Charmaine Min
Roehl, Henry
Monk, Peter N.
Carney, Tom J.
Partridge, Lynda J.
Tetraspanin Cd9b and Cxcl12a/Cxcr4b have a synergistic effect on the control of collective cell migration
description Collective cell migration is essential for embryonic development and homeostatic processes. During zebrafish development, the posterior lateral line primordium (pLLP) navigates along the embryo flank by collective cell migration. The chemokine receptors, Cxcr4b and Cxcr7b, as well as their cognate ligand, Cxcl12a, are essential for this process. We corroborate that knockdown of the zebrafish cd9 tetraspanin orthologue, cd9b, results in mild pLL abnormalities. Through generation of CRISPR and TALEN mutants, we show that cd9a and cd9b function partially redundantly in pLLP migration, which is delayed in the cd9b single and cd9a; cd9b double mutants. This delay led to a transient reduction in neuromast numbers. Loss of both Cd9a and Cd9b sensitized embryos to reduced Cxcr4b and Cxcl12a levels. Together these results provide evidence that Cd9 modulates collective cell migration of the pLLP during zebrafish development. One interpretation of these observations is that Cd9 contributes to more effective chemokine signalling.
author2 Lee Kong Chian School of Medicine (LKCMedicine)
author_facet Lee Kong Chian School of Medicine (LKCMedicine)
Marsay, Katherine S.
Greaves, Sarah
Mahabaleshwar, Harsha
Ho, Charmaine Min
Roehl, Henry
Monk, Peter N.
Carney, Tom J.
Partridge, Lynda J.
format Article
author Marsay, Katherine S.
Greaves, Sarah
Mahabaleshwar, Harsha
Ho, Charmaine Min
Roehl, Henry
Monk, Peter N.
Carney, Tom J.
Partridge, Lynda J.
author_sort Marsay, Katherine S.
title Tetraspanin Cd9b and Cxcl12a/Cxcr4b have a synergistic effect on the control of collective cell migration
title_short Tetraspanin Cd9b and Cxcl12a/Cxcr4b have a synergistic effect on the control of collective cell migration
title_full Tetraspanin Cd9b and Cxcl12a/Cxcr4b have a synergistic effect on the control of collective cell migration
title_fullStr Tetraspanin Cd9b and Cxcl12a/Cxcr4b have a synergistic effect on the control of collective cell migration
title_full_unstemmed Tetraspanin Cd9b and Cxcl12a/Cxcr4b have a synergistic effect on the control of collective cell migration
title_sort tetraspanin cd9b and cxcl12a/cxcr4b have a synergistic effect on the control of collective cell migration
publishDate 2022
url https://hdl.handle.net/10356/163099
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