Integrative multiomics analysis reveals host-microbe-metabolite interplays associated with the aging process in Singaporeans
The age-associated alterations in microbiomes vary across populations due to the influence of genetics and lifestyles. To the best of our knowledge, the microbial changes associated with aging have not yet been investigated in Singapore adults. We conducted shotgun metagenomic sequencing of fecal an...
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sg-ntu-dr.10356-1631432022-11-26T23:31:38Z Integrative multiomics analysis reveals host-microbe-metabolite interplays associated with the aging process in Singaporeans Chen, Liwei Zheng, Tingting Yang, Yifan Chaudhary, Prem Prashant Teh, Jean Pui Yi Cheon, Bobby Kyungbeom Moses, Daniela Schuster, Stephan Christoph Schlundt, Joergen Li, Jun Conway, Patricia Lynne School of Chemical and Biomedical Engineering National Institute of Education School of Social Sciences Nanyang Technological University Food Technology Centre (NAFTEC) Singapore Centre for Environmental Life Sciences and Engineering (SCELSE) Engineering::Bioengineering Microbiome Fecal Metabolome The age-associated alterations in microbiomes vary across populations due to the influence of genetics and lifestyles. To the best of our knowledge, the microbial changes associated with aging have not yet been investigated in Singapore adults. We conducted shotgun metagenomic sequencing of fecal and saliva samples, as well as fecal metabolomics to characterize the gut and oral microbial communities of 62 healthy adult male Singaporeans, including 32 young subjects (age, 23.1 ± 1.4 years) and 30 elderly subjects (age, 69.0 ± 3.5 years). We identified 8 gut and 13 oral species that were differentially abundant in elderly compared to young subjects. By combining the gut and oral microbiomes, 25 age-associated oral-gut species connections were identified. Moreover, oral bacteria Acidaminococcus intestine and Flavonifractor plautii were less prevalent/abundant in elderly gut samples than in young gut samples, whereas Collinsella aerofaciens and Roseburia hominis showed the opposite trends. These results indicate the varied gut-oral communications with aging. Subsequently, we expanded the association studies on microbiome, metabolome and host phenotypic parameters. In particular, Eubacterium eligens increased in elderly compared to young subjects, and was positively correlated with triglycerides, which implies that the potential role of E. eligens in lipid metabolism is altered during the aging process. Our results demonstrated aging-associated changes in the gut and oral microbiomes, as well as the connections between metabolites and host-microbe interactions, thereby deepening the understanding of alterations in the human microbiome during the aging process in a Singapore population. Nanyang Technological University Published version This work was supported by a seed fund from the Nanyang Technological University Integrated Medical, Biological & Environmental Life Sciences (NIMBELS), Singapore (NIM/ 06/2016), Shenzhen Basic Research Program (JCYJ20190808182402941) and Health and Medical Research Fund in Hong Kong (COVID190206). 2022-11-25T01:52:00Z 2022-11-25T01:52:00Z 2022 Journal Article Chen, L., Zheng, T., Yang, Y., Chaudhary, P. P., Teh, J. P. Y., Cheon, B. K., Moses, D., Schuster, S. C., Schlundt, J., Li, J. & Conway, P. L. (2022). Integrative multiomics analysis reveals host-microbe-metabolite interplays associated with the aging process in Singaporeans. Gut Microbes, 14(1), 2070392-. https://dx.doi.org/10.1080/19490976.2022.2070392 1949-0976 https://hdl.handle.net/10356/163143 10.1080/19490976.2022.2070392 35549618 2-s2.0-85130154079 1 14 2070392 en NIM/ 06/2016 Gut Microbes © 2022 The Author(s). Published with license by Taylor & Francis Group, LLC. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. application/pdf |
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Engineering::Bioengineering Microbiome Fecal Metabolome Chen, Liwei Zheng, Tingting Yang, Yifan Chaudhary, Prem Prashant Teh, Jean Pui Yi Cheon, Bobby Kyungbeom Moses, Daniela Schuster, Stephan Christoph Schlundt, Joergen Li, Jun Conway, Patricia Lynne Integrative multiomics analysis reveals host-microbe-metabolite interplays associated with the aging process in Singaporeans |
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The age-associated alterations in microbiomes vary across populations due to the influence of genetics and lifestyles. To the best of our knowledge, the microbial changes associated with aging have not yet been investigated in Singapore adults. We conducted shotgun metagenomic sequencing of fecal and saliva samples, as well as fecal metabolomics to characterize the gut and oral microbial communities of 62 healthy adult male Singaporeans, including 32 young subjects (age, 23.1 ± 1.4 years) and 30 elderly subjects (age, 69.0 ± 3.5 years). We identified 8 gut and 13 oral species that were differentially abundant in elderly compared to young subjects. By combining the gut and oral microbiomes, 25 age-associated oral-gut species connections were identified. Moreover, oral bacteria Acidaminococcus intestine and Flavonifractor plautii were less prevalent/abundant in elderly gut samples than in young gut samples, whereas Collinsella aerofaciens and Roseburia hominis showed the opposite trends. These results indicate the varied gut-oral communications with aging. Subsequently, we expanded the association studies on microbiome, metabolome and host phenotypic parameters. In particular, Eubacterium eligens increased in elderly compared to young subjects, and was positively correlated with triglycerides, which implies that the potential role of E. eligens in lipid metabolism is altered during the aging process. Our results demonstrated aging-associated changes in the gut and oral microbiomes, as well as the connections between metabolites and host-microbe interactions, thereby deepening the understanding of alterations in the human microbiome during the aging process in a Singapore population. |
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School of Chemical and Biomedical Engineering |
author_facet |
School of Chemical and Biomedical Engineering Chen, Liwei Zheng, Tingting Yang, Yifan Chaudhary, Prem Prashant Teh, Jean Pui Yi Cheon, Bobby Kyungbeom Moses, Daniela Schuster, Stephan Christoph Schlundt, Joergen Li, Jun Conway, Patricia Lynne |
format |
Article |
author |
Chen, Liwei Zheng, Tingting Yang, Yifan Chaudhary, Prem Prashant Teh, Jean Pui Yi Cheon, Bobby Kyungbeom Moses, Daniela Schuster, Stephan Christoph Schlundt, Joergen Li, Jun Conway, Patricia Lynne |
author_sort |
Chen, Liwei |
title |
Integrative multiomics analysis reveals host-microbe-metabolite interplays associated with the aging process in Singaporeans |
title_short |
Integrative multiomics analysis reveals host-microbe-metabolite interplays associated with the aging process in Singaporeans |
title_full |
Integrative multiomics analysis reveals host-microbe-metabolite interplays associated with the aging process in Singaporeans |
title_fullStr |
Integrative multiomics analysis reveals host-microbe-metabolite interplays associated with the aging process in Singaporeans |
title_full_unstemmed |
Integrative multiomics analysis reveals host-microbe-metabolite interplays associated with the aging process in Singaporeans |
title_sort |
integrative multiomics analysis reveals host-microbe-metabolite interplays associated with the aging process in singaporeans |
publishDate |
2022 |
url |
https://hdl.handle.net/10356/163143 |
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1751548549956370432 |