Colorimetric and fluorescent TRAP assays for visualising and quantifying fish osteoclast activity

• Main Authors: Ethiraj, Lalith Prabha, Fong, Samuel En Lei, Liu, Ranran, Chan, Madelynn, Winkler, Christoph, Carney, Tom James
• Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
• Format: Article
• Language: English
• Published: 2022
• Subjects: Science::Medicine; Tartrate-Resistant Acid Phosphatase; Osteoclast
• Online Access: https://hdl.handle.net/10356/163218
• Institution: Nanyang Technological University

Histochemical detection of tartrate-resistant acid phosphatase (TRAP) activity is a fundamental technique for visualizing osteoclastic bone resorption and assessing osteoclast activity status in tissues. This approach has mostly employed colorimetric detection, which has limited quantification of ac...