Study on the viability of unprotected bacterial spores directly embedded in a reactive magnesia cement matrix for potential crack healing
Due to the harsh environment of Portland cement (PC), bacteria spores are often protected by porous carriers or encapsulated with soft materials before incorporated in PC concrete for self-healing. However, this often leads to strength reduction of concrete and higher cost. This paper investigated t...
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sg-ntu-dr.10356-1632832022-11-30T03:00:50Z Study on the viability of unprotected bacterial spores directly embedded in a reactive magnesia cement matrix for potential crack healing Xiao, Xi Unluer, Cise Yang, En-Hua School of Civil and Environmental Engineering Engineering::Civil engineering Bacteria Reactive Magnesia Cement Due to the harsh environment of Portland cement (PC), bacteria spores are often protected by porous carriers or encapsulated with soft materials before incorporated in PC concrete for self-healing. However, this often leads to strength reduction of concrete and higher cost. This paper investigated the feasibility of direct addition of unprotected bacteria spores into reactive magnesia cement (RMC) for potential crack healing via microbial-induced carbonate precipitation (MICP) of Bacillus cohnii with magnesium lactate. To examine the bacteria survival and precipitation capability in the matrix, spores were incorporated directly into RMC pastes. Strong MICP evidenced by the massive formation of nesquehonite to fully close the crack was observed in the hardened RMC paste with unprotected bacteria spores addition, which was associated with the high viability of bacteria in dry and low alkaline RMC environment. This study presented a novel finding for the feasibility of the direct incorporation of unprotected bacteria spores for potential crack healing. Ministry of Education (MOE) The authors acknowledge the financial support from the Singapore MOE Academic Research Fund Tier 2 (MOE2017-T2-1-087 (S)). 2022-11-30T03:00:50Z 2022-11-30T03:00:50Z 2022 Journal Article Xiao, X., Unluer, C. & Yang, E. (2022). Study on the viability of unprotected bacterial spores directly embedded in a reactive magnesia cement matrix for potential crack healing. Construction and Building Materials, 346, 128424-. https://dx.doi.org/10.1016/j.conbuildmat.2022.128424 0950-0618 https://hdl.handle.net/10356/163283 10.1016/j.conbuildmat.2022.128424 2-s2.0-85133919205 346 128424 en MOE2017-T2-1-087 (S) Construction and Building Materials © 2022 Elsevier Ltd. All rights reserved. |
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Engineering::Civil engineering Bacteria Reactive Magnesia Cement Xiao, Xi Unluer, Cise Yang, En-Hua Study on the viability of unprotected bacterial spores directly embedded in a reactive magnesia cement matrix for potential crack healing |
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Due to the harsh environment of Portland cement (PC), bacteria spores are often protected by porous carriers or encapsulated with soft materials before incorporated in PC concrete for self-healing. However, this often leads to strength reduction of concrete and higher cost. This paper investigated the feasibility of direct addition of unprotected bacteria spores into reactive magnesia cement (RMC) for potential crack healing via microbial-induced carbonate precipitation (MICP) of Bacillus cohnii with magnesium lactate. To examine the bacteria survival and precipitation capability in the matrix, spores were incorporated directly into RMC pastes. Strong MICP evidenced by the massive formation of nesquehonite to fully close the crack was observed in the hardened RMC paste with unprotected bacteria spores addition, which was associated with the high viability of bacteria in dry and low alkaline RMC environment. This study presented a novel finding for the feasibility of the direct incorporation of unprotected bacteria spores for potential crack healing. |
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School of Civil and Environmental Engineering |
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School of Civil and Environmental Engineering Xiao, Xi Unluer, Cise Yang, En-Hua |
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Article |
author |
Xiao, Xi Unluer, Cise Yang, En-Hua |
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Xiao, Xi |
title |
Study on the viability of unprotected bacterial spores directly embedded in a reactive magnesia cement matrix for potential crack healing |
title_short |
Study on the viability of unprotected bacterial spores directly embedded in a reactive magnesia cement matrix for potential crack healing |
title_full |
Study on the viability of unprotected bacterial spores directly embedded in a reactive magnesia cement matrix for potential crack healing |
title_fullStr |
Study on the viability of unprotected bacterial spores directly embedded in a reactive magnesia cement matrix for potential crack healing |
title_full_unstemmed |
Study on the viability of unprotected bacterial spores directly embedded in a reactive magnesia cement matrix for potential crack healing |
title_sort |
study on the viability of unprotected bacterial spores directly embedded in a reactive magnesia cement matrix for potential crack healing |
publishDate |
2022 |
url |
https://hdl.handle.net/10356/163283 |
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1751548554182131712 |