In-situ scalable manufacturing of Epstein-Barr virus-specific T-cells using bioreactor with an expandable culture area (BECA)

• Main Authors: Chen, Sixun, Ahmad Amirul Bin Abdul Rahim, Wang, Who-Whong, Cheong, Rachael, Prabhu, Akshaya V., Tan, Jerome Zu Yao, Naing, May Win, Toh, Han Chong, Liu, Dan
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Article
• Language: English
• Published: 2022
• Subjects: Engineering::Bioengineering; Bioreactor; Epstein Barr Virus
• Online Access: https://hdl.handle.net/10356/163363
• Institution: Nanyang Technological University

The ex-vivo expansion of antigen-specific T-cells for adoptive T-cell immunotherapy requires active interaction between T-cells and antigen-presenting cells therefore culture density and environment become important variables to control. Maintenance of culture density in a static environment is trad...