Microarray analysis of metabolic changes upon co-expressing periplasmic chaperones in scFvD1.3-producing Escherichia coli.
High expression of recombinant antibody fragments, such as single-chain variable fragments (scFvs), in Escherichia coli imposes an extra metabolic burden on the cells. This would lead to increased rates of protein mis-folding and accumulation of inclusion bodies, generating stress responses which ca...
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| Format: | Final Year Project |
| Language: | English |
| Published: |
2009
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| Online Access: | http://hdl.handle.net/10356/16351 |
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| Institution: | Nanyang Technological University |
| Language: | English |