Squaraine probes for the bimodal staining of lipid droplets and endoplasmic reticulum imaging in live cells
Lipid droplets (LDs) have emerged as a hot target for cancer therapeutics in recent years owing to findings that have shown them to be key organelles involved in maintaining cellular stability and regulating inter-organelle communication through molecular trafficking. LDs emerge from the endoplasmic...
Saved in:
Main Authors: | , , , , , |
---|---|
Other Authors: | |
Format: | Article |
Language: | English |
Published: |
2022
|
Subjects: | |
Online Access: | https://hdl.handle.net/10356/163538 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Nanyang Technological University |
Language: | English |
id |
sg-ntu-dr.10356-163538 |
---|---|
record_format |
dspace |
spelling |
sg-ntu-dr.10356-1635382022-12-08T07:45:34Z Squaraine probes for the bimodal staining of lipid droplets and endoplasmic reticulum imaging in live cells Ferdinandus, Ferdinandus Tan, Jie Ren Lim, Jin Heng Arai, Satoshi Sou, Keitaro Lee, Ken Chi-Lik School of Physical and Mathematical Sciences Science::Biological sciences Fluorescent-Probes Diffusion Lipid droplets (LDs) have emerged as a hot target for cancer therapeutics in recent years owing to findings that have shown them to be key organelles involved in maintaining cellular stability and regulating inter-organelle communication through molecular trafficking. LDs emerge from the endoplasmic reticulum (ER) as a form of cellular homeostasis control. We herein report the study of a library of asymmetric squaraines as superior fluorescence probes to track and image LDs in their native state and environment within cancer cells. The probes are highly selective towards LDs and displayed prominent bright fluorescence with just 1 mu M probe concentration. They also possess bimodal LD and ER staining capability via the simple diffusion of small lipophilic molecules. The probes almost instantly stained LDs, while the ER staining rate is dependent on the probe's lipophilicity and the incubation duration. These "on-demand" organelle-selective probes are highly desirable tools for revealing the role of LDs in governing many cellular processes, especially in malignant cells. Ministry of Education (MOE) This work is supported by the Ministry of Education, Singapore, under its Academic Research Fund Tier 1 (No. RT15/19, to C. L. K. L.). 2022-12-08T07:45:34Z 2022-12-08T07:45:34Z 2022 Journal Article Ferdinandus, F., Tan, J. R., Lim, J. H., Arai, S., Sou, K. & Lee, K. C. (2022). Squaraine probes for the bimodal staining of lipid droplets and endoplasmic reticulum imaging in live cells. Analyst, 147(15), 3570-3577. https://dx.doi.org/10.1039/D2AN00803C 0003-2654 https://hdl.handle.net/10356/163538 10.1039/D2AN00803C 15 147 3570 3577 en RT15/19 Analyst © 2022 The Royal Society of Chemistry. All rights reserved. |
institution |
Nanyang Technological University |
building |
NTU Library |
continent |
Asia |
country |
Singapore Singapore |
content_provider |
NTU Library |
collection |
DR-NTU |
language |
English |
topic |
Science::Biological sciences Fluorescent-Probes Diffusion |
spellingShingle |
Science::Biological sciences Fluorescent-Probes Diffusion Ferdinandus, Ferdinandus Tan, Jie Ren Lim, Jin Heng Arai, Satoshi Sou, Keitaro Lee, Ken Chi-Lik Squaraine probes for the bimodal staining of lipid droplets and endoplasmic reticulum imaging in live cells |
description |
Lipid droplets (LDs) have emerged as a hot target for cancer therapeutics in recent years owing to findings that have shown them to be key organelles involved in maintaining cellular stability and regulating inter-organelle communication through molecular trafficking. LDs emerge from the endoplasmic reticulum (ER) as a form of cellular homeostasis control. We herein report the study of a library of asymmetric squaraines as superior fluorescence probes to track and image LDs in their native state and environment within cancer cells. The probes are highly selective towards LDs and displayed prominent bright fluorescence with just 1 mu M probe concentration. They also possess bimodal LD and ER staining capability via the simple diffusion of small lipophilic molecules. The probes almost instantly stained LDs, while the ER staining rate is dependent on the probe's lipophilicity and the incubation duration. These "on-demand" organelle-selective probes are highly desirable tools for revealing the role of LDs in governing many cellular processes, especially in malignant cells. |
author2 |
School of Physical and Mathematical Sciences |
author_facet |
School of Physical and Mathematical Sciences Ferdinandus, Ferdinandus Tan, Jie Ren Lim, Jin Heng Arai, Satoshi Sou, Keitaro Lee, Ken Chi-Lik |
format |
Article |
author |
Ferdinandus, Ferdinandus Tan, Jie Ren Lim, Jin Heng Arai, Satoshi Sou, Keitaro Lee, Ken Chi-Lik |
author_sort |
Ferdinandus, Ferdinandus |
title |
Squaraine probes for the bimodal staining of lipid droplets and endoplasmic reticulum imaging in live cells |
title_short |
Squaraine probes for the bimodal staining of lipid droplets and endoplasmic reticulum imaging in live cells |
title_full |
Squaraine probes for the bimodal staining of lipid droplets and endoplasmic reticulum imaging in live cells |
title_fullStr |
Squaraine probes for the bimodal staining of lipid droplets and endoplasmic reticulum imaging in live cells |
title_full_unstemmed |
Squaraine probes for the bimodal staining of lipid droplets and endoplasmic reticulum imaging in live cells |
title_sort |
squaraine probes for the bimodal staining of lipid droplets and endoplasmic reticulum imaging in live cells |
publishDate |
2022 |
url |
https://hdl.handle.net/10356/163538 |
_version_ |
1753801155126755328 |