Whole-cell biosynthesis of cytarabine by an unnecessary protein-reduced Escherichia coli that coexpresses purine and uracil phosphorylase

• Main Authors: Li, Ping, Jing, Ruxian, Zhou, Mengping, Jia, Pei, Li, Zhuoya, Liu, Guosheng, Wang, Zhenyu, Wang, Hailei
• Other Authors: Nanyang Environment and Water Research Institute
• Format: Article
• Language: English
• Published: 2022
• Subjects: Science::Biological sciences; Cytarabine; Escherichia Coli
• Online Access: https://hdl.handle.net/10356/163555
• Institution: Nanyang Technological University

Currently, whole-cell catalysts face challenges due to the complexity of reaction systems, although they have a cost advantage over pure enzymes. In this study, cytarabine was synthesized by purified purine phosphorylase 1 (PNP1) and uracil phosphorylase (UP), and the conversion of cytarabine from a...