Enhanced recovery of a biofilm-enabled microbial fuel cell-based biosensor from toxic shocks for wastewater quality monitoring

Microbial fuel cells (MFC) are devices driven by bacterial metabolism and biofilm formation, as has been widely applied in various fields. This is due to its unique capability of sustainable power generation utilizing chemical energy stored in organic wastes. By harnessing bacteria’s ability to resp...

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Main Author: Tang, Nehemiah Sheng En
Other Authors: Cao Bin
Format: Final Year Project
Language:English
Published: Nanyang Technological University 2022
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Online Access:https://hdl.handle.net/10356/163787
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-1637872022-12-17T15:21:43Z Enhanced recovery of a biofilm-enabled microbial fuel cell-based biosensor from toxic shocks for wastewater quality monitoring Tang, Nehemiah Sheng En Cao Bin School of Civil and Environmental Engineering Singapore Centre for Environmental Life Sciences and Engineering (SCELSE) BinCao@ntu.edu.sg Engineering::Environmental engineering Microbial fuel cells (MFC) are devices driven by bacterial metabolism and biofilm formation, as has been widely applied in various fields. This is due to its unique capability of sustainable power generation utilizing chemical energy stored in organic wastes. By harnessing bacteria’s ability to respire and oxidize various electron donors with diverse enzymes, MFCs are often employed in environmental pollution treatment and monitoring. However, multiple limitations to this technology still exist due to its novel nature, such as low sensitivity, short-term performance and low specificity. Hence, research is conducted to hopefully improve upon the current models of MFC-based biosensors, as the technology is shown to be quite promising in terms of providing real-time, in-situ monitoring with a compact nature and is self-powered. Extensive studies have been carried out to increase detection limits and throughput, along with expanding on trace monitoring. In this report, we specifically focused on the “short-term performance” aspect of the device, whereby utilizing recombinant techniques, enhanced versions of microbial strains are engineered and tested to showcase both its improvements and advantages over wild-type strains. The parameters that we focused on were the recovery rate and degree of recovery of MFC-reactors’ voltage outputs post toxic shock/pollutant exposure. Results obtained from this study were non-ideal, as a recovery of the transformed biofilm did not occur and voltage output dropped significantly, the values post-toxic shock were incomparable to pre-toxic shock as there was a huge difference. These results generally indicated that BphS-13 transformed MR-1 did not exhibit enhanced recovery. Bachelor of Engineering (Environmental Engineering) 2022-12-17T15:21:43Z 2022-12-17T15:21:43Z 2022 Final Year Project (FYP) Tang, N. S. E. (2022). Enhanced recovery of a biofilm-enabled microbial fuel cell-based biosensor from toxic shocks for wastewater quality monitoring. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/163787 https://hdl.handle.net/10356/163787 en EN-55 application/pdf Nanyang Technological University
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Environmental engineering
spellingShingle Engineering::Environmental engineering
Tang, Nehemiah Sheng En
Enhanced recovery of a biofilm-enabled microbial fuel cell-based biosensor from toxic shocks for wastewater quality monitoring
description Microbial fuel cells (MFC) are devices driven by bacterial metabolism and biofilm formation, as has been widely applied in various fields. This is due to its unique capability of sustainable power generation utilizing chemical energy stored in organic wastes. By harnessing bacteria’s ability to respire and oxidize various electron donors with diverse enzymes, MFCs are often employed in environmental pollution treatment and monitoring. However, multiple limitations to this technology still exist due to its novel nature, such as low sensitivity, short-term performance and low specificity. Hence, research is conducted to hopefully improve upon the current models of MFC-based biosensors, as the technology is shown to be quite promising in terms of providing real-time, in-situ monitoring with a compact nature and is self-powered. Extensive studies have been carried out to increase detection limits and throughput, along with expanding on trace monitoring. In this report, we specifically focused on the “short-term performance” aspect of the device, whereby utilizing recombinant techniques, enhanced versions of microbial strains are engineered and tested to showcase both its improvements and advantages over wild-type strains. The parameters that we focused on were the recovery rate and degree of recovery of MFC-reactors’ voltage outputs post toxic shock/pollutant exposure. Results obtained from this study were non-ideal, as a recovery of the transformed biofilm did not occur and voltage output dropped significantly, the values post-toxic shock were incomparable to pre-toxic shock as there was a huge difference. These results generally indicated that BphS-13 transformed MR-1 did not exhibit enhanced recovery.
author2 Cao Bin
author_facet Cao Bin
Tang, Nehemiah Sheng En
format Final Year Project
author Tang, Nehemiah Sheng En
author_sort Tang, Nehemiah Sheng En
title Enhanced recovery of a biofilm-enabled microbial fuel cell-based biosensor from toxic shocks for wastewater quality monitoring
title_short Enhanced recovery of a biofilm-enabled microbial fuel cell-based biosensor from toxic shocks for wastewater quality monitoring
title_full Enhanced recovery of a biofilm-enabled microbial fuel cell-based biosensor from toxic shocks for wastewater quality monitoring
title_fullStr Enhanced recovery of a biofilm-enabled microbial fuel cell-based biosensor from toxic shocks for wastewater quality monitoring
title_full_unstemmed Enhanced recovery of a biofilm-enabled microbial fuel cell-based biosensor from toxic shocks for wastewater quality monitoring
title_sort enhanced recovery of a biofilm-enabled microbial fuel cell-based biosensor from toxic shocks for wastewater quality monitoring
publisher Nanyang Technological University
publishDate 2022
url https://hdl.handle.net/10356/163787
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