Synthesis and characterization of novel polyamidoamine for gene delivery
The importance and significance of gene therapy has significantly influence interests in development of novel therapeutic genes as well as promising delivery vectors. Synthetic gene delivery vectors have attracted enormous attention in recent decades because of their ease of manufacturing, targettab...
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sg-ntu-dr.10356-163842023-03-03T15:34:42Z Synthesis and characterization of novel polyamidoamine for gene delivery Goliath Beniah. Chew Sing Yian School of Chemical and Biomedical Engineering A*STAR Institute of Bioengineering and Nanotechnology DRNTU::Engineering::Chemical engineering::Biotechnology The importance and significance of gene therapy has significantly influence interests in development of novel therapeutic genes as well as promising delivery vectors. Synthetic gene delivery vectors have attracted enormous attention in recent decades because of their ease of manufacturing, targettability, and scaling up. However, certain issues like high cytotoxicity and inferior transfection efficiency, like polyethyleneimine (PEI) example, has limited their applications. Still, researchers are still heavily exploiting PEI for cell specific gene delivery. In this project, we developed two novel polyamidoamine containing various types of protonable amines with biodegradable disulfide bonds capable of mediating high gene delivery in mammalian cells namely polymer-1 and polymer-2 (P1 and P2). Both polymers were evaluated for their ability in binding, condensing, and delivering gene to cells. The better of the two, polymer-2, was taken forward to be conjugated with polyethylene glycol (PEG) and targeting ligands for gene delivery to specific cells. The resulting polymer conjugates were able to induce efficient gene delivery in HepG2 cells expressing asialoglycoprotein receptor and 4T1 cells overexpressing folate receptor. The polyamidoamine developed are promising gene delivery carriers for specific cells. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2009-05-26T02:04:58Z 2009-05-26T02:04:58Z 2009 2009 Final Year Project (FYP) http://hdl.handle.net/10356/16384 en Nanyang Technological University 76 p. application/pdf |
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DRNTU::Engineering::Chemical engineering::Biotechnology Goliath Beniah. Synthesis and characterization of novel polyamidoamine for gene delivery |
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The importance and significance of gene therapy has significantly influence interests in development of novel therapeutic genes as well as promising delivery vectors. Synthetic gene delivery vectors have attracted enormous attention in recent decades because of their ease of manufacturing, targettability, and scaling up. However, certain issues like high cytotoxicity and inferior transfection efficiency, like polyethyleneimine (PEI) example, has limited their applications. Still, researchers are still heavily exploiting PEI for cell specific gene delivery. In this project, we developed two novel polyamidoamine containing various types of protonable amines with biodegradable disulfide bonds capable of mediating high gene delivery in mammalian cells namely polymer-1 and polymer-2 (P1 and P2). Both polymers were evaluated for their ability in binding, condensing, and delivering gene to cells. The better of the two, polymer-2, was taken forward to be conjugated with polyethylene glycol (PEG) and targeting ligands for gene delivery to specific cells. The resulting polymer conjugates were able to induce efficient gene delivery in HepG2 cells expressing asialoglycoprotein receptor and 4T1 cells overexpressing folate receptor. The polyamidoamine developed are promising gene delivery carriers for specific cells. |
author2 |
Chew Sing Yian |
author_facet |
Chew Sing Yian Goliath Beniah. |
format |
Final Year Project |
author |
Goliath Beniah. |
author_sort |
Goliath Beniah. |
title |
Synthesis and characterization of novel polyamidoamine for gene delivery |
title_short |
Synthesis and characterization of novel polyamidoamine for gene delivery |
title_full |
Synthesis and characterization of novel polyamidoamine for gene delivery |
title_fullStr |
Synthesis and characterization of novel polyamidoamine for gene delivery |
title_full_unstemmed |
Synthesis and characterization of novel polyamidoamine for gene delivery |
title_sort |
synthesis and characterization of novel polyamidoamine for gene delivery |
publishDate |
2009 |
url |
http://hdl.handle.net/10356/16384 |
_version_ |
1759854600962179072 |