Expression studies on antimicrobial protein, human beta defensin 25 fused with GST
Human β-Defensin 25 (HBD-25) is a novel cationic antimicrobial peptide found in high abundance in male genital tract. The broad spectra of the antimicrobial activity of HBD against microbes make it a potential therapeutic agent in treatment against increasing antibiotic resistance in humans towards...
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| Format: | Final Year Project |
| Language: | English |
| Published: |
2009
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| Online Access: | http://hdl.handle.net/10356/16392 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | Human β-Defensin 25 (HBD-25) is a novel cationic antimicrobial peptide found in high abundance in male genital tract. The broad spectra of the antimicrobial activity of HBD against microbes make it a potential therapeutic agent in treatment against increasing antibiotic resistance in humans towards bacteria infection. Therefore, the expression of high yield and purity of HBD-25 using Ecoli as host cells will be desirable in order to facilitate clinical studies on HBD-25. The harmful effects of the expression of HBD-25 on the Ecoli had been avoided by attaching a fusion tag to the N-terminal of HBD-25. Glutathione S-Transferase (GST) is selected as the fusion tag to enhance the solubility of fusion protein.In this project, the expression conditions required for high yield and purity of GST-HBD-25 were investigated. |
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